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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21871 | 5' | -57.9 | NC_004902.1 | + | 29630 | 0.66 | 0.537628 |
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Target: 5'- --gAGCUACagGGCGGCaGGUgugccaucuGCCAgGGa -3' miRNA: 3'- ucaUCGAUGa-CCGCUG-CCG---------CGGUgCC- -5' |
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| 21871 | 5' | -57.9 | NC_004902.1 | + | 36614 | 0.67 | 0.445759 |
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Target: 5'- --cGGCUGCUGGCGGCacUGCCcuugcaacagguACGGg -3' miRNA: 3'- ucaUCGAUGACCGCUGccGCGG------------UGCC- -5' |
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| 21871 | 5' | -57.9 | NC_004902.1 | + | 20280 | 0.7 | 0.30692 |
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Target: 5'- aAGUGGUUGC-GGCGGCGGauucuccgaUGCCugGa -3' miRNA: 3'- -UCAUCGAUGaCCGCUGCC---------GCGGugCc -5' |
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| 21871 | 5' | -57.9 | NC_004902.1 | + | 843 | 0.74 | 0.168828 |
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Target: 5'- uGUAGCUGgcugugugaaUGGCGAUGGCuacCCACGGg -3' miRNA: 3'- uCAUCGAUg---------ACCGCUGCCGc--GGUGCC- -5' |
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| 21871 | 5' | -57.9 | NC_004902.1 | + | 36653 | 1.09 | 0.000427 |
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Target: 5'- cAGUAGCUACUGGCGACGGCGCCACGGc -3' miRNA: 3'- -UCAUCGAUGACCGCUGCCGCGGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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