Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21872 | 3' | -54.1 | NC_004902.1 | + | 17711 | 0.67 | 0.612312 |
Target: 5'- aUCAGUUGaGGcAAUCUGGUG-AUGAGu -3' miRNA: 3'- aGGUCGACgCC-UUAGACCACgUACUC- -5' |
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21872 | 3' | -54.1 | NC_004902.1 | + | 34433 | 0.67 | 0.600932 |
Target: 5'- cUCCAGUUGCGGGAUCaucUGCGaGAc -3' miRNA: 3'- -AGGUCGACGCCUUAGaccACGUaCUc -5' |
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21872 | 3' | -54.1 | NC_004902.1 | + | 33774 | 0.68 | 0.567001 |
Target: 5'- gCCAGC-GCGG---CUGGUugGCAUGGGa -3' miRNA: 3'- aGGUCGaCGCCuuaGACCA--CGUACUC- -5' |
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21872 | 3' | -54.1 | NC_004902.1 | + | 455 | 0.69 | 0.51169 |
Target: 5'- aCCuGCUGCGGuuUCuuggUGGUGCccuUGGGu -3' miRNA: 3'- aGGuCGACGCCuuAG----ACCACGu--ACUC- -5' |
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21872 | 3' | -54.1 | NC_004902.1 | + | 11838 | 0.71 | 0.380593 |
Target: 5'- gUUguGcCUGCGG-AUCUGGUGCGgaUGGGu -3' miRNA: 3'- -AGguC-GACGCCuUAGACCACGU--ACUC- -5' |
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21872 | 3' | -54.1 | NC_004902.1 | + | 36876 | 1.09 | 0.000912 |
Target: 5'- gUCCAGCUGCGGAAUCUGGUGCAUGAGc -3' miRNA: 3'- -AGGUCGACGCCUUAGACCACGUACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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