Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21877 | 3' | -54.9 | NC_004902.1 | + | 11097 | 0.66 | 0.698031 |
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Target: 5'- cUCUGAUuaagagUGGCAAUGCagCG-GGUguuacgACCCa -3' miRNA: 3'- -GGACUA------ACCGUUACGg-GCaCCG------UGGG- -5' |
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| 21877 | 3' | -54.9 | NC_004902.1 | + | 5822 | 0.66 | 0.698031 |
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Target: 5'- aCCUGAUUGGCucggGCC----GCACCg -3' miRNA: 3'- -GGACUAACCGuua-CGGgcacCGUGGg -5' |
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| 21877 | 3' | -54.9 | NC_004902.1 | + | 20811 | 0.67 | 0.620089 |
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Target: 5'- aCCUGg--GGaCGAcGCCCGUguGGCAUUCa -3' miRNA: 3'- -GGACuaaCC-GUUaCGGGCA--CCGUGGG- -5' |
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| 21877 | 3' | -54.9 | NC_004902.1 | + | 25516 | 0.67 | 0.597759 |
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Target: 5'- aCCUGGUgcacUGGgAA-GCCCGuUGGCGuugUCCa -3' miRNA: 3'- -GGACUA----ACCgUUaCGGGC-ACCGU---GGG- -5' |
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| 21877 | 3' | -54.9 | NC_004902.1 | + | 43708 | 0.67 | 0.586635 |
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Target: 5'- aUUGAUgauGCAAUGCCUGuUGGUcauuCCCg -3' miRNA: 3'- gGACUAac-CGUUACGGGC-ACCGu---GGG- -5' |
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| 21877 | 3' | -54.9 | NC_004902.1 | + | 2085 | 0.68 | 0.579979 |
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Target: 5'- aUCUGAggcUGGCuugaaaGAUGCCUGUgccacuucaauggucGGCGCCa -3' miRNA: 3'- -GGACUa--ACCG------UUACGGGCA---------------CCGUGGg -5' |
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| 21877 | 3' | -54.9 | NC_004902.1 | + | 6334 | 0.7 | 0.428688 |
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Target: 5'- cCCUGuggGGUc-UGCCgGUGGUugCCa -3' miRNA: 3'- -GGACuaaCCGuuACGGgCACCGugGG- -5' |
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| 21877 | 3' | -54.9 | NC_004902.1 | + | 6298 | 1.14 | 0.000367 |
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Target: 5'- aCCUGAUUGGCAAUGCCCGUGGCACCCu -3' miRNA: 3'- -GGACUAACCGUUACGGGCACCGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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