Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21878 | 3' | -55.4 | NC_004902.1 | + | 15753 | 0.66 | 0.631999 |
Target: 5'- uGGCgUGCCAGACagcugGCCGguAGCc-- -3' miRNA: 3'- gCUGgGCGGUCUGa----CGGCguUUGuuu -5' |
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21878 | 3' | -55.4 | NC_004902.1 | + | 8434 | 0.66 | 0.620676 |
Target: 5'- -uGCCCGCCAGACgacgGCCaucAACAc- -3' miRNA: 3'- gcUGGGCGGUCUGa---CGGcguUUGUuu -5' |
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21878 | 3' | -55.4 | NC_004902.1 | + | 19255 | 0.66 | 0.609363 |
Target: 5'- aGACCCG-CAGugUGCCuGCAc----- -3' miRNA: 3'- gCUGGGCgGUCugACGG-CGUuuguuu -5' |
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21878 | 3' | -55.4 | NC_004902.1 | + | 21274 | 0.66 | 0.586809 |
Target: 5'- aGAUcgCCGCaAGGCagGCCGCAGACGc- -3' miRNA: 3'- gCUG--GGCGgUCUGa-CGGCGUUUGUuu -5' |
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21878 | 3' | -55.4 | NC_004902.1 | + | 15329 | 0.67 | 0.564416 |
Target: 5'- aGGCgCCGCCAGcCUGacuaucgacguCCGUGAACAAc -3' miRNA: 3'- gCUG-GGCGGUCuGAC-----------GGCGUUUGUUu -5' |
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21878 | 3' | -55.4 | NC_004902.1 | + | 11949 | 0.69 | 0.436945 |
Target: 5'- aGGCUCuaCAGAUUGCCGCGGAa--- -3' miRNA: 3'- gCUGGGcgGUCUGACGGCGUUUguuu -5' |
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21878 | 3' | -55.4 | NC_004902.1 | + | 5494 | 0.7 | 0.3982 |
Target: 5'- -cGCCCG-CAGGCUGUCGguGACAu- -3' miRNA: 3'- gcUGGGCgGUCUGACGGCguUUGUuu -5' |
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21878 | 3' | -55.4 | NC_004902.1 | + | 40041 | 0.7 | 0.398199 |
Target: 5'- -uACCCGacguaaCCuGGCUGUCGCAGGCAAGa -3' miRNA: 3'- gcUGGGC------GGuCUGACGGCGUUUGUUU- -5' |
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21878 | 3' | -55.4 | NC_004902.1 | + | 10479 | 1.06 | 0.00098 |
Target: 5'- uCGACCCGCCAGACUGCCGCAAACAAAa -3' miRNA: 3'- -GCUGGGCGGUCUGACGGCGUUUGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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