Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21878 | 5' | -52 | NC_004902.1 | + | 26726 | 0.66 | 0.781925 |
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Target: 5'- uGgCUGCUccacacaCGGCAGGUAGuagGCCg- -3' miRNA: 3'- uCgGACGAa------GCCGUCCAUUua-UGGau -5' |
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| 21878 | 5' | -52 | NC_004902.1 | + | 32714 | 0.68 | 0.681361 |
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Target: 5'- cAGCCgcuaGCUgagUGGCAGGgcucuGUGCCa- -3' miRNA: 3'- -UCGGa---CGAa--GCCGUCCauu--UAUGGau -5' |
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| 21878 | 5' | -52 | NC_004902.1 | + | 5439 | 0.68 | 0.669742 |
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Target: 5'- cAGCCUGCg--GGCGuuuGGUGAgcGUGCCg- -3' miRNA: 3'- -UCGGACGaagCCGU---CCAUU--UAUGGau -5' |
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| 21878 | 5' | -52 | NC_004902.1 | + | 7912 | 0.7 | 0.564826 |
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Target: 5'- uGCCUGUguaUUGGCaagAGGgcgGGAUGCCUGa -3' miRNA: 3'- uCGGACGa--AGCCG---UCCa--UUUAUGGAU- -5' |
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| 21878 | 5' | -52 | NC_004902.1 | + | 36248 | 0.7 | 0.564826 |
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Target: 5'- aAGCCgugGCUUCcccuGCGGGUGcagGGUACCUu -3' miRNA: 3'- -UCGGa--CGAAGc---CGUCCAU---UUAUGGAu -5' |
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| 21878 | 5' | -52 | NC_004902.1 | + | 26088 | 0.71 | 0.497125 |
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Target: 5'- gGGCCUGCauuugUCaGCAgGGUGAGUGCUg- -3' miRNA: 3'- -UCGGACGa----AGcCGU-CCAUUUAUGGau -5' |
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| 21878 | 5' | -52 | NC_004902.1 | + | 39618 | 0.72 | 0.443623 |
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Target: 5'- gGGCCUGCcaaGGCAGGgaguGUugCUGg -3' miRNA: 3'- -UCGGACGaagCCGUCCauu-UAugGAU- -5' |
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| 21878 | 5' | -52 | NC_004902.1 | + | 2664 | 0.72 | 0.423144 |
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Target: 5'- cGCCUGCgaaUCGGUcguugcggAGGUucAUGCCUAu -3' miRNA: 3'- uCGGACGa--AGCCG--------UCCAuuUAUGGAU- -5' |
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| 21878 | 5' | -52 | NC_004902.1 | + | 10517 | 1.07 | 0.001734 |
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Target: 5'- cAGCCUGCUUCGGCAGGUAAAUACCUAc -3' miRNA: 3'- -UCGGACGAAGCCGUCCAUUUAUGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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