Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21881 | 5' | -52.9 | NC_004902.1 | + | 17961 | 0.66 | 0.747471 |
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Target: 5'- uUGGCUGgGCAGCUuGGuuGGCGGagUGCa -3' miRNA: 3'- -ACCGACaUGUCGAcUCu-CCGUUg-AUG- -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 31858 | 0.66 | 0.73644 |
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Target: 5'- gUGGUUGUgGgAGCgUGAGGGGguGCaGCu -3' miRNA: 3'- -ACCGACA-UgUCG-ACUCUCCguUGaUG- -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 4479 | 0.67 | 0.668273 |
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Target: 5'- cUGGCUGUAgUAGCUGcuucGGGCAgACUGu -3' miRNA: 3'- -ACCGACAU-GUCGACuc--UCCGU-UGAUg -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 22740 | 0.67 | 0.668273 |
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Target: 5'- gGGCUGUcaGCGGCggGGGuGGUGGCg-- -3' miRNA: 3'- aCCGACA--UGUCGa-CUCuCCGUUGaug -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 25648 | 0.67 | 0.656704 |
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Target: 5'- uUGGCUacagACAGCUGccaAGcAGGCAAUUAa -3' miRNA: 3'- -ACCGAca--UGUCGAC---UC-UCCGUUGAUg -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 4485 | 0.67 | 0.64511 |
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Target: 5'- gGGCUGcccaAGCUaGGAGGUAauGCUGCc -3' miRNA: 3'- aCCGACaug-UCGAcUCUCCGU--UGAUG- -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 16417 | 0.69 | 0.519103 |
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Target: 5'- gUGGggGUACAGCgGucguuuGAuGGCAGCUGCu -3' miRNA: 3'- -ACCgaCAUGUCGaCu-----CU-CCGUUGAUG- -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 14865 | 0.7 | 0.497079 |
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Target: 5'- aGGCgcauaugGUGCAGU--GGcGGCAACUGCg -3' miRNA: 3'- aCCGa------CAUGUCGacUCuCCGUUGAUG- -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 10808 | 0.71 | 0.413724 |
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Target: 5'- aGGUaagccuCAGCucagUGAGGGGCAGCUGCg -3' miRNA: 3'- aCCGacau--GUCG----ACUCUCCGUUGAUG- -5' |
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| 21881 | 5' | -52.9 | NC_004902.1 | + | 18261 | 1.11 | 0.000729 |
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Target: 5'- uUGGCUGUACAGCUGAGAGGCAACUACa -3' miRNA: 3'- -ACCGACAUGUCGACUCUCCGUUGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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