Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21882 | 3' | -54.2 | NC_004902.1 | + | 39568 | 0.66 | 0.744958 |
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Target: 5'- cCUCCA-GGUGCgGGUgcaugGCAAGGACu-- -3' miRNA: 3'- -GAGGUgCCAUGgUCG-----UGUUCCUGucc -5' |
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| 21882 | 3' | -54.2 | NC_004902.1 | + | 35911 | 0.66 | 0.744958 |
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Target: 5'- cCUCCugGuUGCUGGUcgaACAAGGgcaACAGGu -3' miRNA: 3'- -GAGGugCcAUGGUCG---UGUUCC---UGUCC- -5' |
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| 21882 | 3' | -54.2 | NC_004902.1 | + | 16567 | 0.66 | 0.72348 |
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Target: 5'- -cCCACauaGGcauUGCCAGCugAAGGAggaGGGa -3' miRNA: 3'- gaGGUG---CC---AUGGUCGugUUCCUg--UCC- -5' |
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| 21882 | 3' | -54.2 | NC_004902.1 | + | 22877 | 0.71 | 0.420493 |
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Target: 5'- gUCCACGG-AgCAGCuuucguuGCAGGGuCAGGa -3' miRNA: 3'- gAGGUGCCaUgGUCG-------UGUUCCuGUCC- -5' |
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| 21882 | 3' | -54.2 | NC_004902.1 | + | 12293 | 0.72 | 0.366014 |
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Target: 5'- gCUCCGCGGcccUACaGGCGCAGGGuGCAGc -3' miRNA: 3'- -GAGGUGCC---AUGgUCGUGUUCC-UGUCc -5' |
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| 21882 | 3' | -54.2 | NC_004902.1 | + | 13483 | 0.81 | 0.097196 |
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Target: 5'- uUUCACGGUGCgGGCAuugaucCAGGGACAGGc -3' miRNA: 3'- gAGGUGCCAUGgUCGU------GUUCCUGUCC- -5' |
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| 21882 | 3' | -54.2 | NC_004902.1 | + | 21004 | 0.94 | 0.012388 |
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Target: 5'- cCUCCACGGU-CaCAGCACAAGGACAGGc -3' miRNA: 3'- -GAGGUGCCAuG-GUCGUGUUCCUGUCC- -5' |
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| 21882 | 3' | -54.2 | NC_004902.1 | + | 21106 | 0.94 | 0.012388 |
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Target: 5'- cCUCCACGGU-CaCAGCACAAGGACAGGc -3' miRNA: 3'- -GAGGUGCCAuG-GUCGUGUUCCUGUCC- -5' |
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| 21882 | 3' | -54.2 | NC_004902.1 | + | 21208 | 0.94 | 0.012388 |
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Target: 5'- cCUCCACGGU-CaCAGCACAAGGACAGGc -3' miRNA: 3'- -GAGGUGCCAuG-GUCGUGUUCCUGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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