Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21885 | 3' | -58.5 | NC_004902.1 | + | 22385 | 0.66 | 0.446895 |
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Target: 5'- gGGGC-GAGUAUUCuaaguGGCUGGCAa-- -3' miRNA: 3'- gCCUGaCUCGUGAGu----CCGACCGUcgu -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 36720 | 0.66 | 0.427361 |
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Target: 5'- cCGGGCgcgGuAGCugUCAgcaccGGCUcccgcGGCAGCGc -3' miRNA: 3'- -GCCUGa--C-UCGugAGU-----CCGA-----CCGUCGU- -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 657 | 0.66 | 0.417785 |
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Target: 5'- aGGAUggcgGAGCAgUUguuGGGaaGGCAGCAc -3' miRNA: 3'- gCCUGa---CUCGUgAG---UCCgaCCGUCGU- -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 42702 | 0.66 | 0.416835 |
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Target: 5'- uGGcucgucuGCUGuuacuAGCugUCAGGCUGGCGuuGCc -3' miRNA: 3'- gCC-------UGAC-----UCGugAGUCCGACCGU--CGu -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 4213 | 0.67 | 0.408341 |
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Target: 5'- -uGAUUGGGCACagCAGuGCUGGCAcCAc -3' miRNA: 3'- gcCUGACUCGUGa-GUC-CGACCGUcGU- -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 15753 | 0.7 | 0.255982 |
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Target: 5'- uGGACUG-GCACUCAGuGgaGGauuGCAg -3' miRNA: 3'- gCCUGACuCGUGAGUC-CgaCCgu-CGU- -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 22915 | 0.7 | 0.236682 |
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Target: 5'- aGGACuUGAGUA---AGGCUGaGCAGCAg -3' miRNA: 3'- gCCUG-ACUCGUgagUCCGAC-CGUCGU- -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 1856 | 0.72 | 0.19113 |
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Target: 5'- gGGGgUGAGCACUCAccauauguuGGCUGacCAGCAa -3' miRNA: 3'- gCCUgACUCGUGAGU---------CCGACc-GUCGU- -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 15255 | 0.75 | 0.115889 |
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Target: 5'- aCGGACgucGA-UAgUCAGGCUGGCGGCGc -3' miRNA: 3'- -GCCUGa--CUcGUgAGUCCGACCGUCGU- -5' |
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| 21885 | 3' | -58.5 | NC_004902.1 | + | 24309 | 1.08 | 0.000321 |
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Target: 5'- gCGGACUGAGCACUCAGGCUGGCAGCAa -3' miRNA: 3'- -GCCUGACUCGUGAGUCCGACCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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