Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2189 | 3' | -48.6 | NC_001396.1 | + | 1453 | 0.66 | 0.438906 |
Target: 5'- gGCaGcgCAAGGaaggcCACCAGCAGAcCACGu -3' miRNA: 3'- aCG-CuaGUUCU-----GUGGUUGUCUaGUGU- -5' |
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2189 | 3' | -48.6 | NC_001396.1 | + | 5014 | 0.72 | 0.192289 |
Target: 5'- aUGCGGUauuGGGCGCUGACAugcgcGAUCGCAc -3' miRNA: 3'- -ACGCUAgu-UCUGUGGUUGU-----CUAGUGU- -5' |
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2189 | 3' | -48.6 | NC_001396.1 | + | 672 | 0.73 | 0.168288 |
Target: 5'- gGCaugGAUCAGGaACACCAACAcGAUCAa- -3' miRNA: 3'- aCG---CUAGUUC-UGUGGUUGU-CUAGUgu -5' |
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2189 | 3' | -48.6 | NC_001396.1 | + | 5820 | 1.1 | 0.000224 |
Target: 5'- uUGCGAUCAAGACACCAACAGAUCACAg -3' miRNA: 3'- -ACGCUAGUUCUGUGGUUGUCUAGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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