Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21890 | 5' | -56.1 | NC_004902.1 | + | 43974 | 0.66 | 0.645418 |
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Target: 5'- gUUGACC-CUgGUCACUG-GAGUGGg -3' miRNA: 3'- aGAUUGGuGGgCAGUGGCuCUCGCCg -5' |
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| 21890 | 5' | -56.1 | NC_004902.1 | + | 4650 | 0.67 | 0.590314 |
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Target: 5'- ---uGCCugCUG-CACCaGAGGGCaGGCu -3' miRNA: 3'- agauUGGugGGCaGUGG-CUCUCG-CCG- -5' |
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| 21890 | 5' | -56.1 | NC_004902.1 | + | 21369 | 0.68 | 0.504368 |
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Target: 5'- gUCUGcugcGCCGCCUGUaguGCCGcGucuGCGGCc -3' miRNA: 3'- -AGAU----UGGUGGGCAg--UGGCuCu--CGCCG- -5' |
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| 21890 | 5' | -56.1 | NC_004902.1 | + | 5414 | 0.69 | 0.463416 |
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Target: 5'- --aAGCCACCCuggccaauGUCACCGAcAGCcugcgGGCg -3' miRNA: 3'- agaUUGGUGGG--------CAGUGGCUcUCG-----CCG- -5' |
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| 21890 | 5' | -56.1 | NC_004902.1 | + | 36099 | 0.72 | 0.289374 |
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Target: 5'- aCUGACCGCCUGUaCACaCGGGcAGUcacuGGCa -3' miRNA: 3'- aGAUUGGUGGGCA-GUG-GCUC-UCG----CCG- -5' |
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| 21890 | 5' | -56.1 | NC_004902.1 | + | 27741 | 1.13 | 0.000376 |
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Target: 5'- uUCUAACCACCCGUCACCGAGAGCGGCg -3' miRNA: 3'- -AGAUUGGUGGGCAGUGGCUCUCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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