Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21891 | 3' | -52.8 | NC_004902.1 | + | 12921 | 0.7 | 0.483242 |
Target: 5'- -uGGCuCUGCGAguGCAGCUggUGCAg -3' miRNA: 3'- ugUCGcGAUGUUguCGUCGGuaACGU- -5' |
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21891 | 3' | -52.8 | NC_004902.1 | + | 214 | 0.7 | 0.472479 |
Target: 5'- -gGGCGCUGCGuCAGgGGCCuuaGCAg -3' miRNA: 3'- ugUCGCGAUGUuGUCgUCGGuaaCGU- -5' |
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21891 | 3' | -52.8 | NC_004902.1 | + | 36636 | 0.71 | 0.4307 |
Target: 5'- --cGCGCUGCcGCGGgAGCCggUGCu -3' miRNA: 3'- uguCGCGAUGuUGUCgUCGGuaACGu -5' |
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21891 | 3' | -52.8 | NC_004902.1 | + | 4379 | 0.71 | 0.417594 |
Target: 5'- uCAGCGCaaugcuuugcucaaUGCGGC-GCAGCCAggGCGa -3' miRNA: 3'- uGUCGCG--------------AUGUUGuCGUCGGUaaCGU- -5' |
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21891 | 3' | -52.8 | NC_004902.1 | + | 18838 | 0.72 | 0.372309 |
Target: 5'- cACAG-GC-ACA--GGCAGCCAUUGCAa -3' miRNA: 3'- -UGUCgCGaUGUugUCGUCGGUAACGU- -5' |
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21891 | 3' | -52.8 | NC_004902.1 | + | 32124 | 0.77 | 0.174347 |
Target: 5'- uACGGCGCaGguGCAGCAGCaAUUGCAg -3' miRNA: 3'- -UGUCGCGaUguUGUCGUCGgUAACGU- -5' |
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21891 | 3' | -52.8 | NC_004902.1 | + | 29834 | 1.08 | 0.001087 |
Target: 5'- uACAGCGCUACAACAGCAGCCAUUGCAu -3' miRNA: 3'- -UGUCGCGAUGUUGUCGUCGGUAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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