Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21891 | 5' | -55.1 | NC_004902.1 | + | 38287 | 0.66 | 0.641006 |
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Target: 5'- -gUUCAGCGcGCCGCagaGCCuGUAGUg -3' miRNA: 3'- aaAAGUCGC-CGGUGaagUGG-CGUCGg -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 37645 | 0.66 | 0.608014 |
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Target: 5'- --aUCAGCGGCUACguaaacgcauuuggcUCACagcgggGCAGCg -3' miRNA: 3'- aaaAGUCGCCGGUGa--------------AGUGg-----CGUCGg -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 5414 | 0.67 | 0.572958 |
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Target: 5'- ----aAGCcacccuGGCCAaugUCACCGaCAGCCu -3' miRNA: 3'- aaaagUCG------CCGGUga-AGUGGC-GUCGG- -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 16425 | 0.67 | 0.561742 |
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Target: 5'- ---aCAGCGGUCGUUUgAUgGCAGCUg -3' miRNA: 3'- aaaaGUCGCCGGUGAAgUGgCGUCGG- -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 10315 | 0.67 | 0.550587 |
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Target: 5'- -cUUUGGCGGCCGCacgaGCgGUGGCUg -3' miRNA: 3'- aaAAGUCGCCGGUGaag-UGgCGUCGG- -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 13119 | 0.67 | 0.539501 |
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Target: 5'- --gUUGGCGGUUAUucuccaggacuUUCACCaauGCAGCCa -3' miRNA: 3'- aaaAGUCGCCGGUG-----------AAGUGG---CGUCGG- -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 19029 | 0.67 | 0.538397 |
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Target: 5'- -----uGCaGCCGCUUUACCgucgacgGCAGCCu -3' miRNA: 3'- aaaaguCGcCGGUGAAGUGG-------CGUCGG- -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 24399 | 0.67 | 0.528492 |
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Target: 5'- --cUUGGCGGCaCGCUgCGCCuuGCuGCCa -3' miRNA: 3'- aaaAGUCGCCG-GUGAaGUGG--CGuCGG- -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 39304 | 0.73 | 0.250313 |
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Target: 5'- --aUCAGCcuaGGCUACUUCACCaccaucgaagagGCAGCa -3' miRNA: 3'- aaaAGUCG---CCGGUGAAGUGG------------CGUCGg -5' |
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| 21891 | 5' | -55.1 | NC_004902.1 | + | 29890 | 1.08 | 0.00069 |
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Target: 5'- cUUUUCAGCGGCCACUUCACCGCAGCCc -3' miRNA: 3'- -AAAAGUCGCCGGUGAAGUGGCGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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