miRNA: 3'- aCUGUGAC-GGCGG---------UUUGUCGuu--CCGU- -5'
miRNA: 3'- aCUGUgACGGCGGuUUGUCGuuCCGu -5'
miRNA: 3'- aCUgu---GACGGCGGU--UUGUCGUuCCGU- -5'
miRNA: 3'- aCUGUGA---------CGG----------CGgUUUGUCGUUC-CGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21892 | 5' | -54.4 | NC_004902.1 | + | 36103 | 0.72 | 0.325133 |
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Target: 5'- cGACACUGaCCGCCuguacacacGGGCAGUcacuGGCAu -3' miRNA: 3'- aCUGUGAC-GGCGG---------UUUGUCGuu--CCGU- -5' |
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| 21892 | 5' | -54.4 | NC_004902.1 | + | 36551 | 0.71 | 0.350649 |
|
Target: 5'- gGGCAgUGCCGCC-AGCAGCc-GGUu -3' miRNA: 3'- aCUGUgACGGCGGuUUGUCGuuCCGu -5' |
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| 21892 | 5' | -54.4 | NC_004902.1 | + | 37258 | 0.71 | 0.377557 |
|
Target: 5'- cGAaauccCUGCCGCCAccAACGGCGuGGUAu -3' miRNA: 3'- aCUgu---GACGGCGGU--UUGUCGUuCCGU- -5' |
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| 21892 | 5' | -54.4 | NC_004902.1 | + | 40498 | 0.67 | 0.564317 |
|
Target: 5'- cGACACUuuugaggaaGCCaucgcagcgcGCgAAGCAGCAAGgGCAg -3' miRNA: 3'- aCUGUGA---------CGG----------CGgUUUGUCGUUC-CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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