miRNA: 3'- -ACUG--UGACGGCGgUU------UGUCGu--------------UCCGU- -5'
miRNA: 3'- aCUGUGACGG------------CGGUU-UG---UCGUUCCGu -5'
miRNA: 3'- aCUGUGaCGGcggUUUGUCGUUCCGU- -5'
miRNA: 3'- -ACUGUGACGGCGGUUUGUCGUUCCGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21892 | 5' | -54.4 | NC_004902.1 | + | 19358 | 0.68 | 0.509911 |
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Target: 5'- uUGACagACUGCCGCgGAuccuugGCAGCuuuguugcugccucuGGGCAg -3' miRNA: 3'- -ACUG--UGACGGCGgUU------UGUCGu--------------UCCGU- -5' |
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| 21892 | 5' | -54.4 | NC_004902.1 | + | 28939 | 0.68 | 0.553066 |
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Target: 5'- gGGCACUGCCacaucugccuccGUCAAcACcuuGGCAAGGCc -3' miRNA: 3'- aCUGUGACGG------------CGGUU-UG---UCGUUCCGu -5' |
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| 21892 | 5' | -54.4 | NC_004902.1 | + | 2415 | 0.67 | 0.564317 |
|
Target: 5'- aGGCAUgGCUugaAGACGGCAGGGCAa -3' miRNA: 3'- aCUGUGaCGGcggUUUGUCGUUCCGU- -5' |
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| 21892 | 5' | -54.4 | NC_004902.1 | + | 32933 | 1.09 | 0.000703 |
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Target: 5'- uUGACACUGCCGCCAAACAGCAAGGCAg -3' miRNA: 3'- -ACUGUGACGGCGGUUUGUCGUUCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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