Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21899 | 3' | -51.6 | NC_004902.1 | + | 14664 | 0.66 | 0.812411 |
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Target: 5'- uGGCCAG---GCAaAAGCUGUGCcGGGa -3' miRNA: 3'- -UCGGUCauaCGUcUUCGGUACGuUCC- -5' |
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| 21899 | 3' | -51.6 | NC_004902.1 | + | 24041 | 0.66 | 0.802364 |
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Target: 5'- -cUCGGUGUGCGGAAcGCCGcUGcCGAGa -3' miRNA: 3'- ucGGUCAUACGUCUU-CGGU-AC-GUUCc -5' |
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| 21899 | 3' | -51.6 | NC_004902.1 | + | 6617 | 0.66 | 0.792116 |
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Target: 5'- uGGCaaugGUGCAGAGGCacuUGCAGGc -3' miRNA: 3'- -UCGgucaUACGUCUUCGgu-ACGUUCc -5' |
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| 21899 | 3' | -51.6 | NC_004902.1 | + | 22889 | 0.67 | 0.781681 |
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Target: 5'- gAGCCAGUGUGauaAGuGGCU-UGCucAGGa -3' miRNA: 3'- -UCGGUCAUACg--UCuUCGGuACGu-UCC- -5' |
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| 21899 | 3' | -51.6 | NC_004902.1 | + | 26533 | 0.67 | 0.738335 |
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Target: 5'- uGCCAG-AUGCGGAugcGCCAcGCuggcugAAGGa -3' miRNA: 3'- uCGGUCaUACGUCUu--CGGUaCG------UUCC- -5' |
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| 21899 | 3' | -51.6 | NC_004902.1 | + | 36649 | 0.69 | 0.658385 |
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Target: 5'- gAGCCGGUGcUGaCAGcuaccgcGCCcgGUGCGAGGg -3' miRNA: 3'- -UCGGUCAU-AC-GUCuu-----CGG--UACGUUCC- -5' |
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| 21899 | 3' | -51.6 | NC_004902.1 | + | 10065 | 0.81 | 0.128063 |
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Target: 5'- uGGCCAGgaaccaugAUGCAGuAGCCAUGCuAGGc -3' miRNA: 3'- -UCGGUCa-------UACGUCuUCGGUACGuUCC- -5' |
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| 21899 | 3' | -51.6 | NC_004902.1 | + | 39457 | 1.11 | 0.001146 |
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Target: 5'- gAGCCAGUAUGCAGAAGCCAUGCAAGGc -3' miRNA: 3'- -UCGGUCAUACGUCUUCGGUACGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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