Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 219 | 3' | -53.8 | AC_000008.1 | + | 17714 | 0.66 | 0.69081 |
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Target: 5'- uCCCUCAGcaucuGGcCUGUGCUGCUgUUCc -3' miRNA: 3'- -GGGAGUUu----UCaGACGUGACGGgGAGc -5' |
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| 219 | 3' | -53.8 | AC_000008.1 | + | 33728 | 0.66 | 0.67947 |
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Target: 5'- uUCCUCcugcugCUGCuGCUGCUCCUCc -3' miRNA: 3'- -GGGAGuuuucaGACG-UGACGGGGAGc -5' |
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| 219 | 3' | -53.8 | AC_000008.1 | + | 25812 | 0.66 | 0.656656 |
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Target: 5'- aCCUUu-GAG-CUGCAggGUCCCUCGc -3' miRNA: 3'- gGGAGuuUUCaGACGUgaCGGGGAGC- -5' |
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| 219 | 3' | -53.8 | AC_000008.1 | + | 26342 | 0.68 | 0.531742 |
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Target: 5'- aCCCUC---GGUC-GCAUU-CCCCUCGc -3' miRNA: 3'- -GGGAGuuuUCAGaCGUGAcGGGGAGC- -5' |
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| 219 | 3' | -53.8 | AC_000008.1 | + | 26413 | 0.69 | 0.477562 |
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Target: 5'- cUCCUCAGgcGcCgccgGCACUGCCCgUUCGc -3' miRNA: 3'- -GGGAGUUuuCaGa---CGUGACGGG-GAGC- -5' |
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| 219 | 3' | -53.8 | AC_000008.1 | + | 23964 | 0.69 | 0.467049 |
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Target: 5'- gCgCUCGGGgguGGUUUcGCGCUGCUCCUCu -3' miRNA: 3'- -GgGAGUUU---UCAGA-CGUGACGGGGAGc -5' |
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| 219 | 3' | -53.8 | AC_000008.1 | + | 30356 | 1.1 | 0.000642 |
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Target: 5'- gCCCUCAAAAGUCUGCACUGCCCCUCGu -3' miRNA: 3'- -GGGAGUUUUCAGACGUGACGGGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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