Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
219 | 5' | -57.3 | AC_000008.1 | + | 15173 | 0.66 | 0.477149 |
Target: 5'- -gCCAcacGGGCugagGAGaaGCGCGCUGAGGc -3' miRNA: 3'- gaGGUu--CCCG----UUCgaCGCGCGGUUCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 6646 | 0.66 | 0.466802 |
Target: 5'- --gCGGGcGGCAAGC-GCGCGCUcguauGGGu -3' miRNA: 3'- gagGUUC-CCGUUCGaCGCGCGGu----UCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 11381 | 0.66 | 0.456574 |
Target: 5'- uUCCAcgcAGGGCGcgAGCUGCG-GCau-GGc -3' miRNA: 3'- gAGGU---UCCCGU--UCGACGCgCGguuCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 11415 | 0.66 | 0.456574 |
Target: 5'- aUCgCGAGcGGUu-GCUGCGCGagGAGGa -3' miRNA: 3'- gAG-GUUC-CCGuuCGACGCGCggUUCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 4318 | 0.66 | 0.446467 |
Target: 5'- uUUCAGuaGCAAGCUGauUGCCAGGGg -3' miRNA: 3'- gAGGUUccCGUUCGACgcGCGGUUCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 27297 | 0.66 | 0.446467 |
Target: 5'- cCUCCGGGGGCucGCc-CGCGUCAc-- -3' miRNA: 3'- -GAGGUUCCCGuuCGacGCGCGGUucc -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 10777 | 0.66 | 0.436486 |
Target: 5'- uUCCAGGcGCGgcGGCUGCuGCGCUAGc- -3' miRNA: 3'- gAGGUUCcCGU--UCGACG-CGCGGUUcc -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 18354 | 0.67 | 0.416916 |
Target: 5'- uCUCCAgaGGGGCGuggcgaaaAGCgucCGCGCCccgacAGGGa -3' miRNA: 3'- -GAGGU--UCCCGU--------UCGac-GCGCGG-----UUCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 23391 | 0.67 | 0.416916 |
Target: 5'- -gCguGGGGUuuGC-GCGCGCCGGGa -3' miRNA: 3'- gaGguUCCCGuuCGaCGCGCGGUUCc -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 7914 | 0.67 | 0.379434 |
Target: 5'- uCUCCcuGGGCGGuaagccggacGCcGUGCGCCGGGc -3' miRNA: 3'- -GAGGuuCCCGUU----------CGaCGCGCGGUUCc -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 8459 | 0.68 | 0.370422 |
Target: 5'- uUCCAGGGGCugguuGGUgGCGgCGUCGAuGGc -3' miRNA: 3'- gAGGUUCCCGu----UCGaCGC-GCGGUU-CC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 8656 | 0.68 | 0.344277 |
Target: 5'- -gCCGcgcgcGGGCAGgagcugguGCUGCGCGCguAGGu -3' miRNA: 3'- gaGGUu----CCCGUU--------CGACGCGCGguUCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 12440 | 0.68 | 0.335861 |
Target: 5'- ---gAAGGGCAuggaGGCUGCGCGCgCGcugauGGa -3' miRNA: 3'- gaggUUCCCGU----UCGACGCGCG-GUu----CC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 5340 | 0.68 | 0.335028 |
Target: 5'- ---aAGGGGUgcgcuccGGGCUGCGCgcugGCCAGGGu -3' miRNA: 3'- gaggUUCCCG-------UUCGACGCG----CGGUUCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 20229 | 0.69 | 0.303715 |
Target: 5'- --gUggGGGCuGGCggGCGCGCCAAa- -3' miRNA: 3'- gagGuuCCCGuUCGa-CGCGCGGUUcc -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 25254 | 0.69 | 0.296056 |
Target: 5'- -cUCAAGGGCGAG--GCGCGCCGc-- -3' miRNA: 3'- gaGGUUCCCGUUCgaCGCGCGGUucc -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 5576 | 0.7 | 0.246635 |
Target: 5'- uUCCGGGGaGUAGGCaucCGCGCCGcAGGc -3' miRNA: 3'- gAGGUUCC-CGUUCGac-GCGCGGU-UCC- -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 10887 | 0.71 | 0.209893 |
Target: 5'- cCUCCAAGucGGCAGGCUcGCGCGUUugAAGc -3' miRNA: 3'- -GAGGUUC--CCGUUCGA-CGCGCGG--UUCc -5' |
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219 | 5' | -57.3 | AC_000008.1 | + | 30393 | 1.11 | 0.000222 |
Target: 5'- cCUCCAAGGGCAAGCUGCGCGCCAAGGg -3' miRNA: 3'- -GAGGUUCCCGUUCGACGCGCGGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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