Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21905 | 3' | -48.4 | NC_004913.1 | + | 59607 | 0.66 | 0.980716 |
Target: 5'- uGCUCcggaacaACUGGCgcugCGCCGGucgcauucugaAGGUGACCa -3' miRNA: 3'- -UGAG-------UGACUGaa--GUGGCU-----------UUCGUUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 19533 | 0.66 | 0.975784 |
Target: 5'- -aUCACUGACagccUUACCGGuuGCcuucaGACCu -3' miRNA: 3'- ugAGUGACUGa---AGUGGCUuuCG-----UUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 19421 | 0.67 | 0.962498 |
Target: 5'- -gUUACUGAUacugUCGguaaagguCUGAAGGCAACCg -3' miRNA: 3'- ugAGUGACUGa---AGU--------GGCUUUCGUUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 45721 | 0.67 | 0.958509 |
Target: 5'- cGCUCAuUUGGCgaggUCAUgGAAgacuggguuAGCGACCu -3' miRNA: 3'- -UGAGU-GACUGa---AGUGgCUU---------UCGUUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 54339 | 0.67 | 0.958509 |
Target: 5'- cGC-CugUGGCUUCugcACCGgAAGGCGAUg -3' miRNA: 3'- -UGaGugACUGAAG---UGGC-UUUCGUUGg -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 1221 | 0.68 | 0.934239 |
Target: 5'- aGCUCAUgaGCUgaUCAUC-AAAGCAGCCu -3' miRNA: 3'- -UGAGUGacUGA--AGUGGcUUUCGUUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 16798 | 0.68 | 0.934239 |
Target: 5'- cCUU-CUGACcagaACCGAGAGCAGCa -3' miRNA: 3'- uGAGuGACUGaag-UGGCUUUCGUUGg -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 45379 | 0.69 | 0.928492 |
Target: 5'- cGCUCACUG-CUgucaUCAuCCaGAAGCAAUCc -3' miRNA: 3'- -UGAGUGACuGA----AGU-GGcUUUCGUUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 19865 | 0.7 | 0.879898 |
Target: 5'- uACUCugaGCUGGCgcagUCACCGAAauuucAGCAGa- -3' miRNA: 3'- -UGAG---UGACUGa---AGUGGCUU-----UCGUUgg -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 33912 | 0.7 | 0.879898 |
Target: 5'- uGCUCACUGugUggcggaaaUCAUgGcAAAGCcGCCa -3' miRNA: 3'- -UGAGUGACugA--------AGUGgC-UUUCGuUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 8014 | 0.71 | 0.816858 |
Target: 5'- gACggCGCUGACaguUUCACCGGAugcgcucAGCGGCa -3' miRNA: 3'- -UGa-GUGACUG---AAGUGGCUU-------UCGUUGg -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 51776 | 0.72 | 0.808026 |
Target: 5'- cCUCACUGACgcagUCugUGGcaaGAGCGAUg -3' miRNA: 3'- uGAGUGACUGa---AGugGCU---UUCGUUGg -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 8541 | 0.73 | 0.745408 |
Target: 5'- cGCUgCugUGACUgCACgGGucGCAGCCu -3' miRNA: 3'- -UGA-GugACUGAaGUGgCUuuCGUUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 55293 | 0.75 | 0.655388 |
Target: 5'- gGCUgACUGACUggCGCUGAuuuucucAGCGGCCc -3' miRNA: 3'- -UGAgUGACUGAa-GUGGCUu------UCGUUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 4256 | 0.76 | 0.552484 |
Target: 5'- cGCUCACUGAacgUUUCGCUG-AAGUGGCCc -3' miRNA: 3'- -UGAGUGACU---GAAGUGGCuUUCGUUGG- -5' |
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21905 | 3' | -48.4 | NC_004913.1 | + | 4381 | 1.13 | 0.003073 |
Target: 5'- gACUCACUGACUUCACCGAAAGCAACCu -3' miRNA: 3'- -UGAGUGACUGAAGUGGCUUUCGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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