Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21906 | 5' | -54.6 | NC_004913.1 | + | 25249 | 0.66 | 0.750048 |
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Target: 5'- aACGGUAGgCGUucUGcCAG-GCAUCCa -3' miRNA: 3'- gUGCCGUCgGUA--ACuGUCuCGUAGGc -5' |
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| 21906 | 5' | -54.6 | NC_004913.1 | + | 5757 | 0.66 | 0.727864 |
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Target: 5'- uCACGGCGugauuGCCGUUGAUAcAGCAgugaagcUCUGa -3' miRNA: 3'- -GUGCCGU-----CGGUAACUGUcUCGU-------AGGC- -5' |
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| 21906 | 5' | -54.6 | NC_004913.1 | + | 55944 | 0.67 | 0.694366 |
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Target: 5'- aACGGCAGCCGauaaaccauaGCGGAGUuUCCu -3' miRNA: 3'- gUGCCGUCGGUaac-------UGUCUCGuAGGc -5' |
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| 21906 | 5' | -54.6 | NC_004913.1 | + | 9588 | 0.7 | 0.510526 |
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Target: 5'- uGCGGCAgGCCcg-GugGGGGCGaCCGg -3' miRNA: 3'- gUGCCGU-CGGuaaCugUCUCGUaGGC- -5' |
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| 21906 | 5' | -54.6 | NC_004913.1 | + | 17600 | 0.71 | 0.45908 |
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Target: 5'- gGgGGCGGCUAUUGGC---GCAUCCGu -3' miRNA: 3'- gUgCCGUCGGUAACUGucuCGUAGGC- -5' |
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| 21906 | 5' | -54.6 | NC_004913.1 | + | 6890 | 1.08 | 0.001312 |
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Target: 5'- cCACGGCAGCCAUUGACAGAGCAUCCGu -3' miRNA: 3'- -GUGCCGUCGGUAACUGUCUCGUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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