Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21907 | 5' | -59.2 | NC_004913.1 | + | 8573 | 0.66 | 0.488646 |
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Target: 5'- -gCCAGGCUgGCGCaggagAGCG-CCGGa- -3' miRNA: 3'- aaGGUCCGAaCGCGg----UCGUaGGCCag -5' |
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| 21907 | 5' | -59.2 | NC_004913.1 | + | 59520 | 0.66 | 0.488646 |
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Target: 5'- -aCC-GGCgcaGCGCCAGUuguUCCGGa- -3' miRNA: 3'- aaGGuCCGaa-CGCGGUCGu--AGGCCag -5' |
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| 21907 | 5' | -59.2 | NC_004913.1 | + | 8659 | 0.66 | 0.468487 |
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Target: 5'- cUCCGGcGCUcuccUGCGCCAGC--CUGGcCu -3' miRNA: 3'- aAGGUC-CGA----ACGCGGUCGuaGGCCaG- -5' |
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| 21907 | 5' | -59.2 | NC_004913.1 | + | 9798 | 0.66 | 0.448763 |
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Target: 5'- gUCC-GGUgaugagUGCGCCAucuGUAUCCGGg- -3' miRNA: 3'- aAGGuCCGa-----ACGCGGU---CGUAGGCCag -5' |
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| 21907 | 5' | -59.2 | NC_004913.1 | + | 25552 | 0.68 | 0.369588 |
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Target: 5'- uUUCCuGGUccUUGCGCuCugcaaucguccgcauGGUAUCCGGUCu -3' miRNA: 3'- -AAGGuCCG--AACGCG-G---------------UCGUAGGCCAG- -5' |
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| 21907 | 5' | -59.2 | NC_004913.1 | + | 45092 | 0.68 | 0.366145 |
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Target: 5'- cUCC-GGCuUUGCGCCAGaaCGUUCGGaUCu -3' miRNA: 3'- aAGGuCCG-AACGCGGUC--GUAGGCC-AG- -5' |
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| 21907 | 5' | -59.2 | NC_004913.1 | + | 22958 | 0.69 | 0.312576 |
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Target: 5'- aUCCAGaGaauggaaccaucgUGCGCCAGCGcacCCGGUCc -3' miRNA: 3'- aAGGUC-Cga-----------ACGCGGUCGUa--GGCCAG- -5' |
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| 21907 | 5' | -59.2 | NC_004913.1 | + | 7509 | 1.06 | 0.000508 |
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Target: 5'- uUUCCAGGCUUGCGCCAGCAUCCGGUCu -3' miRNA: 3'- -AAGGUCCGAACGCGGUCGUAGGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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