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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 21908 | 3' | -48.1 | NC_004913.1 | + | 8270 | 1.08 | 0.00627 |
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Target: 5'- cGUAACAGCAUAUUGAGCGUCACCGUAc -3' miRNA: 3'- -CAUUGUCGUAUAACUCGCAGUGGCAU- -5' |
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| 21908 | 3' | -48.1 | NC_004913.1 | + | 16052 | 0.79 | 0.389266 |
|
Target: 5'- aGUAACAGCGU--UGAGCGUUACCu-- -3' miRNA: 3'- -CAUUGUCGUAuaACUCGCAGUGGcau -5' |
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| 21908 | 3' | -48.1 | NC_004913.1 | + | 50684 | 0.68 | 0.936171 |
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Target: 5'- -gGAuCGGUAUGUUGAGCGUgaauUGCCGg- -3' miRNA: 3'- caUU-GUCGUAUAACUCGCA----GUGGCau -5' |
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| 21908 | 3' | -48.1 | NC_004913.1 | + | 1785 | 0.67 | 0.946734 |
|
Target: 5'- aGUGACuGCcgAUggcUGAuGCGUCGCCGc- -3' miRNA: 3'- -CAUUGuCGuaUA---ACU-CGCAGUGGCau -5' |
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| 21908 | 3' | -48.1 | NC_004913.1 | + | 26598 | 0.67 | 0.959889 |
|
Target: 5'- uGUGGCGGCGgg-UGAccugacgGCGUC-CCGUGc -3' miRNA: 3'- -CAUUGUCGUauaACU-------CGCAGuGGCAU- -5' |
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| 21908 | 3' | -48.1 | NC_004913.1 | + | 6728 | 0.67 | 0.960298 |
|
Target: 5'- -gAACAGCAUGgcggaacUGAGUGauuuuUUACCGUAu -3' miRNA: 3'- caUUGUCGUAUa------ACUCGC-----AGUGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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