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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21908 | 5' | -51 | NC_004913.1 | + | 454 | 0.66 | 0.932284 |
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Target: 5'- uCCCUCA--ACGcCACaggaggcguuucugACGUCCGgacGACg -3' miRNA: 3'- -GGGAGUuaUGC-GUG--------------UGCAGGCau-CUG- -5' |
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| 21908 | 5' | -51 | NC_004913.1 | + | 1264 | 0.68 | 0.879722 |
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Target: 5'- aCCCUCAccggcauucugGCGCGCAUcUCC-UGGGCa -3' miRNA: 3'- -GGGAGUua---------UGCGUGUGcAGGcAUCUG- -5' |
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| 21908 | 5' | -51 | NC_004913.1 | + | 20911 | 0.69 | 0.811961 |
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Target: 5'- uUCUCAAUGCGUuccGCugGUCU--GGACg -3' miRNA: 3'- gGGAGUUAUGCG---UGugCAGGcaUCUG- -5' |
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| 21908 | 5' | -51 | NC_004913.1 | + | 16835 | 0.7 | 0.751584 |
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Target: 5'- gCCUCucuuGUugGCGCugGUCUGggugcGGCa -3' miRNA: 3'- gGGAGu---UAugCGUGugCAGGCau---CUG- -5' |
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| 21908 | 5' | -51 | NC_004913.1 | + | 8235 | 1.13 | 0.001795 |
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Target: 5'- cCCCUCAAUACGCACACGUCCGUAGACa -3' miRNA: 3'- -GGGAGUUAUGCGUGUGCAGGCAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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