Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21909 | 5' | -52.5 | NC_004913.1 | + | 57648 | 0.73 | 0.503548 |
Target: 5'- -cCUGCGGAUCAacgCCACCuGC-UCCg -3' miRNA: 3'- cuGACGCCUAGUguaGGUGG-UGuAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 53506 | 0.72 | 0.524824 |
Target: 5'- ---gGCGGcUCAUGUCCugCACAUCa -3' miRNA: 3'- cugaCGCCuAGUGUAGGugGUGUAGg -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 46702 | 0.69 | 0.723229 |
Target: 5'- gGACUGCGGuuccUCGCGUUCACgucggCGCAUUUc -3' miRNA: 3'- -CUGACGCCu---AGUGUAGGUG-----GUGUAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 32481 | 0.67 | 0.842548 |
Target: 5'- --gUGCGGcaauuucaccguGUCGCAUCCcuguaaaaacaGCCACuGUCCa -3' miRNA: 3'- cugACGCC------------UAGUGUAGG-----------UGGUG-UAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 25424 | 0.66 | 0.867976 |
Target: 5'- cGAUgGCGGAUCAgCgAUUCGCuCAgGUCCu -3' miRNA: 3'- -CUGaCGCCUAGU-G-UAGGUG-GUgUAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 24481 | 0.68 | 0.79379 |
Target: 5'- cGCUGacaccacCGGAUCACcauuuuuAUCCACgGCAUCa -3' miRNA: 3'- cUGAC-------GCCUAGUG-------UAGGUGgUGUAGg -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 21554 | 0.67 | 0.815079 |
Target: 5'- --gUGCuG-UCGCGUUCugCGCAUCCg -3' miRNA: 3'- cugACGcCuAGUGUAGGugGUGUAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 21414 | 0.7 | 0.634966 |
Target: 5'- -gUUGUGGuaaaACGaCCACCACGUCCg -3' miRNA: 3'- cuGACGCCuag-UGUaGGUGGUGUAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 20564 | 0.73 | 0.46219 |
Target: 5'- aGCUGCGGAugcgUCACccUCUguuGCCAUAUCCg -3' miRNA: 3'- cUGACGCCU----AGUGu-AGG---UGGUGUAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 20389 | 0.69 | 0.733966 |
Target: 5'- -uCUGCGGccUgACGUCUGCCACGauaUCCg -3' miRNA: 3'- cuGACGCCu-AgUGUAGGUGGUGU---AGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 19990 | 0.66 | 0.851263 |
Target: 5'- uGCUGCGGGUCGgCGUCaauggACaaaaACGUCUg -3' miRNA: 3'- cUGACGCCUAGU-GUAGg----UGg---UGUAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 18763 | 0.66 | 0.875957 |
Target: 5'- --aUGCGGuugugaaccaGUCGCAgcauaauauucaUCCACCGCAgCCu -3' miRNA: 3'- cugACGCC----------UAGUGU------------AGGUGGUGUaGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 14990 | 0.66 | 0.854683 |
Target: 5'- -cCUGUGGAuuuuuauccuugccuUCAUAUCCACUACccgaCCa -3' miRNA: 3'- cuGACGCCU---------------AGUGUAGGUGGUGua--GG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 13656 | 0.68 | 0.795762 |
Target: 5'- cAUUGCGGAUCACcaGUCC--UACAUUCu -3' miRNA: 3'- cUGACGCCUAGUG--UAGGugGUGUAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 11068 | 1.13 | 0.001162 |
Target: 5'- gGACUGCGGAUCACAUCCACCACAUCCg -3' miRNA: 3'- -CUGACGCCUAGUGUAGGUGGUGUAGG- -5' |
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21909 | 5' | -52.5 | NC_004913.1 | + | 5009 | 0.73 | 0.503548 |
Target: 5'- cGCUGCuGAcucaUACGUCCGCCuGCAUCCa -3' miRNA: 3'- cUGACGcCUa---GUGUAGGUGG-UGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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