Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21912 | 5' | -56.3 | NC_004913.1 | + | 44358 | 0.66 | 0.677064 |
Target: 5'- uCUAGCCga-AGCCaGAGAGGCAagaaggcuacaacaaCCGCa -3' miRNA: 3'- -GGUCGGuagUCGG-CUUUCCGUg--------------GGUG- -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 47869 | 0.66 | 0.675977 |
Target: 5'- uUCAGCCGccgCAGCaacuGAu-GGUGCCCAUg -3' miRNA: 3'- -GGUCGGUa--GUCGg---CUuuCCGUGGGUG- -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 18365 | 0.66 | 0.665094 |
Target: 5'- aCCAGCg--CAGCagGAAGGGUACgUACu -3' miRNA: 3'- -GGUCGguaGUCGg-CUUUCCGUGgGUG- -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 26696 | 0.66 | 0.665094 |
Target: 5'- aCCGGCag-CAcgggacGCCGucAGGuCACCCGCc -3' miRNA: 3'- -GGUCGguaGU------CGGCuuUCC-GUGGGUG- -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 1877 | 0.66 | 0.642153 |
Target: 5'- uCCAGCggcgacgCAUCAGCC-AucGGCAgUCACu -3' miRNA: 3'- -GGUCG-------GUAGUCGGcUuuCCGUgGGUG- -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 56676 | 0.67 | 0.599509 |
Target: 5'- aCAGgaAUcCAGCCcacaGAAAGGCACCgACa -3' miRNA: 3'- gGUCggUA-GUCGG----CUUUCCGUGGgUG- -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 20500 | 0.67 | 0.588621 |
Target: 5'- gCAGCuCAUC-GCCGGGAaGCugCCAg -3' miRNA: 3'- gGUCG-GUAGuCGGCUUUcCGugGGUg -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 19614 | 0.67 | 0.577769 |
Target: 5'- uUCGGUCAUCGGCguauucagcgcaCGAAcuGCACCCGg -3' miRNA: 3'- -GGUCGGUAGUCG------------GCUUucCGUGGGUg -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 55460 | 0.68 | 0.513852 |
Target: 5'- aCCGGCagcUCcggAGCCGGAAGGCAUCgCGg -3' miRNA: 3'- -GGUCGgu-AG---UCGGCUUUCCGUGG-GUg -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 15796 | 0.71 | 0.364553 |
Target: 5'- cCCcGUCAacugguugcugagguUCAGCCGAuauGGCACCCGu -3' miRNA: 3'- -GGuCGGU---------------AGUCGGCUuu-CCGUGGGUg -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 9610 | 0.73 | 0.304985 |
Target: 5'- aCCGGaCC-UCaggGGCCGAAGGGCgACCCGg -3' miRNA: 3'- -GGUC-GGuAG---UCGGCUUUCCG-UGGGUg -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 55220 | 0.74 | 0.236768 |
Target: 5'- gCCAGUCAgUCAGCCGGAAGcaaACCCGa -3' miRNA: 3'- -GGUCGGU-AGUCGGCUUUCcg-UGGGUg -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 52690 | 0.76 | 0.181809 |
Target: 5'- aCCAgGCCGaacUCA-CCGGGAGGCACCCGg -3' miRNA: 3'- -GGU-CGGU---AGUcGGCUUUCCGUGGGUg -5' |
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21912 | 5' | -56.3 | NC_004913.1 | + | 15565 | 1.12 | 0.000522 |
Target: 5'- uCCAGCCAUCAGCCGAAAGGCACCCACg -3' miRNA: 3'- -GGUCGGUAGUCGGCUUUCCGUGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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