Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21916 | 3' | -56.2 | NC_004913.1 | + | 3183 | 0.69 | 0.432376 |
Target: 5'- cGCCGCCAgGGCAcaUGCAGCU-GCAc -3' miRNA: 3'- -CGGCGGUaCCGUaaGUGUCGGuCGUu -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 6821 | 0.8 | 0.080349 |
Target: 5'- gGCUGCCGUGGCAUUCAgCcGCCAGUc- -3' miRNA: 3'- -CGGCGGUACCGUAAGU-GuCGGUCGuu -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 17611 | 0.67 | 0.579343 |
Target: 5'- cGCCGCCAUcuGUuuGUUCGCAGCCu-CAAu -3' miRNA: 3'- -CGGCGGUAc-CG--UAAGUGUCGGucGUU- -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 20105 | 1.08 | 0.000808 |
Target: 5'- uGCCGCCAUGGCAUUCACAGCCAGCAAu -3' miRNA: 3'- -CGGCGGUACCGUAAGUGUCGGUCGUU- -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 26234 | 0.66 | 0.612656 |
Target: 5'- cGCCGCCugcUGGCGUg-ACAaaCCGGCAu -3' miRNA: 3'- -CGGCGGu--ACCGUAagUGUc-GGUCGUu -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 35924 | 0.69 | 0.44219 |
Target: 5'- cGCCGCCAUacGGUAUU-GCAGCggcacgacgagCAGCAAa -3' miRNA: 3'- -CGGCGGUA--CCGUAAgUGUCG-----------GUCGUU- -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 36051 | 0.67 | 0.579343 |
Target: 5'- aGCgCGCCGcUGGCAUUCGCAucaaaagagGUgAGUAAu -3' miRNA: 3'- -CG-GCGGU-ACCGUAAGUGU---------CGgUCGUU- -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 39110 | 0.69 | 0.44219 |
Target: 5'- uUCGCCAgacGGCAUUUAaaggugauGCCAGCGAu -3' miRNA: 3'- cGGCGGUa--CCGUAAGUgu------CGGUCGUU- -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 48004 | 0.68 | 0.482655 |
Target: 5'- aGgUGCUAUGGCAUggUCaACGGCUGGCGc -3' miRNA: 3'- -CgGCGGUACCGUA--AG-UGUCGGUCGUu -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 54609 | 0.72 | 0.272556 |
Target: 5'- -aCGCCAuaacauacUGGU--UCGCAGCCAGCAc -3' miRNA: 3'- cgGCGGU--------ACCGuaAGUGUCGGUCGUu -5' |
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21916 | 3' | -56.2 | NC_004913.1 | + | 56751 | 0.66 | 0.590414 |
Target: 5'- gGCUgGCC-UGGCAaUCACAGUUGGCu- -3' miRNA: 3'- -CGG-CGGuACCGUaAGUGUCGGUCGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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