Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21918 | 3' | -51.9 | NC_004913.1 | + | 45984 | 0.66 | 0.900289 |
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Target: 5'- uUGAUUgcCAUCUGGCuGUAUaGCGUCGu -3' miRNA: 3'- -ACUAAaaGUAGGCCG-CAUG-CGCGGUg -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 38340 | 0.66 | 0.893119 |
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Target: 5'- gGAUaa-UGUCCGGUGUcAUGCaGCCACc -3' miRNA: 3'- aCUAaaaGUAGGCCGCA-UGCG-CGGUG- -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 22429 | 0.67 | 0.861737 |
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Target: 5'- gGAUUUugUCAUCCGGUG-ACgGUGCUg- -3' miRNA: 3'- aCUAAA--AGUAGGCCGCaUG-CGCGGug -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 3133 | 0.67 | 0.835552 |
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Target: 5'- ---aUUUCAUCCGGUGauuUugGCGUCc- -3' miRNA: 3'- acuaAAAGUAGGCCGC---AugCGCGGug -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 45101 | 0.68 | 0.816963 |
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Target: 5'- gGGUUaUCAcUCCGGCuu-UGCGCCAg -3' miRNA: 3'- aCUAAaAGU-AGGCCGcauGCGCGGUg -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 27014 | 0.69 | 0.746109 |
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Target: 5'- aGAaUUUCAgcgauUCCGGUGUGauauccacCGCGCCGg -3' miRNA: 3'- aCUaAAAGU-----AGGCCGCAU--------GCGCGGUg -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 24172 | 0.72 | 0.546413 |
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Target: 5'- cGGUaaaCAUCgGGCGUACGcCGCCGg -3' miRNA: 3'- aCUAaaaGUAGgCCGCAUGC-GCGGUg -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 3797 | 0.74 | 0.471772 |
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Target: 5'- ------gCAUCCGGCGUcgGUGCCACa -3' miRNA: 3'- acuaaaaGUAGGCCGCAugCGCGGUG- -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 53847 | 0.74 | 0.46152 |
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Target: 5'- gUGAUUUg---CCGGCGUAC-CGCCAa -3' miRNA: 3'- -ACUAAAaguaGGCCGCAUGcGCGGUg -5' |
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| 21918 | 3' | -51.9 | NC_004913.1 | + | 21385 | 1.1 | 0.002031 |
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Target: 5'- cUGAUUUUCAUCCGGCGUACGCGCCACu -3' miRNA: 3'- -ACUAAAAGUAGGCCGCAUGCGCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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