Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21919 | 3' | -52.9 | NC_004913.1 | + | 12618 | 0.66 | 0.858398 |
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Target: 5'- uGCAUAUgaGGcACCACCCGAUGuucgacgccgGGCGa -3' miRNA: 3'- -UGUGUGggCUaUGGUGGGCUAU----------UCGU- -5' |
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| 21919 | 3' | -52.9 | NC_004913.1 | + | 55811 | 0.67 | 0.774043 |
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Target: 5'- aGCACGCCUauUGCCGCCCacUGAGaCGg -3' miRNA: 3'- -UGUGUGGGcuAUGGUGGGcuAUUC-GU- -5' |
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| 21919 | 3' | -52.9 | NC_004913.1 | + | 12048 | 0.69 | 0.655319 |
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Target: 5'- aACACAUCCugcuUACCAUCCGGUuucguguaacAGGCAa -3' miRNA: 3'- -UGUGUGGGcu--AUGGUGGGCUA----------UUCGU- -5' |
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| 21919 | 3' | -52.9 | NC_004913.1 | + | 21049 | 0.72 | 0.533616 |
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Target: 5'- gUAUugCUGAUGCUgACCCGAUgGAGCAg -3' miRNA: 3'- uGUGugGGCUAUGG-UGGGCUA-UUCGU- -5' |
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| 21919 | 3' | -52.9 | NC_004913.1 | + | 13401 | 0.72 | 0.533616 |
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Target: 5'- uGC-CACCUGAUACCGCaaaCGgAUAGGCu -3' miRNA: 3'- -UGuGUGGGCUAUGGUGg--GC-UAUUCGu -5' |
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| 21919 | 3' | -52.9 | NC_004913.1 | + | 52787 | 0.73 | 0.450245 |
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Target: 5'- uGCAUgguGCCgGGUGCCuCCCGGUGAGUu -3' miRNA: 3'- -UGUG---UGGgCUAUGGuGGGCUAUUCGu -5' |
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| 21919 | 3' | -52.9 | NC_004913.1 | + | 22226 | 1.09 | 0.001985 |
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Target: 5'- uACACACCCGAUACCACCCGAUAAGCAg -3' miRNA: 3'- -UGUGUGGGCUAUGGUGGGCUAUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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