Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21920 | 3' | -60.4 | NC_004913.1 | + | 8909 | 0.7 | 0.273319 |
Target: 5'- --cGCuCCCCCUGUucgcccuucUCACCACG-CGGa -3' miRNA: 3'- aguCGcGGGGGACG---------AGUGGUGCuGCC- -5' |
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21920 | 3' | -60.4 | NC_004913.1 | + | 9202 | 0.66 | 0.460037 |
Target: 5'- gCAGCGCUgCCUGCcggaccuggcUCuCCACGA-GGu -3' miRNA: 3'- aGUCGCGGgGGACG----------AGuGGUGCUgCC- -5' |
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21920 | 3' | -60.4 | NC_004913.1 | + | 19443 | 0.73 | 0.169267 |
Target: 5'- aUCAGC-CCCCCUGCU-GCCAcCGGCa- -3' miRNA: 3'- -AGUCGcGGGGGACGAgUGGU-GCUGcc -5' |
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21920 | 3' | -60.4 | NC_004913.1 | + | 26170 | 0.7 | 0.280018 |
Target: 5'- gCGGCGUCUCUUGC-CAaaaaCAUGACGGu -3' miRNA: 3'- aGUCGCGGGGGACGaGUg---GUGCUGCC- -5' |
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21920 | 3' | -60.4 | NC_004913.1 | + | 26293 | 1.1 | 0.000344 |
Target: 5'- aUCAGCGCCCCCUGCUCACCACGACGGu -3' miRNA: 3'- -AGUCGCGGGGGACGAGUGGUGCUGCC- -5' |
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21920 | 3' | -60.4 | NC_004913.1 | + | 56416 | 0.69 | 0.338315 |
Target: 5'- cCAGCGCgCUCUcCUgGUCACGACGGg -3' miRNA: 3'- aGUCGCGgGGGAcGAgUGGUGCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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