miRNA: 3'- gUGGCAGUacAAAAACCGUU-CUcUGC- -5'
miRNA: 3'- -GUGGCAGUACAAaaacCGuUCUCUGc -5'
miRNA: 3'- gUGGCAGUACaaaaAC-CGUUCUCUGC- -5'
miRNA: 3'- -GUGGCAGUACAAAAACCGUUCUCUGC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21920 | 5' | -47.2 | NC_004913.1 | + | 5143 | 0.69 | 0.940643 |
|
Target: 5'- gACCGUCAcuUUUUUGGCGGuGAuGCGa -3' miRNA: 3'- gUGGCAGUacAAAAACCGUU-CUcUGC- -5' |
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| 21920 | 5' | -47.2 | NC_004913.1 | + | 6706 | 0.68 | 0.967049 |
|
Target: 5'- uCGCCGUCGUGUU----GC-GGAGACu -3' miRNA: 3'- -GUGGCAGUACAAaaacCGuUCUCUGc -5' |
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| 21920 | 5' | -47.2 | NC_004913.1 | + | 26206 | 0.79 | 0.505757 |
|
Target: 5'- aACCGUCGUGg---UGaGCAGGGGGCGc -3' miRNA: 3'- gUGGCAGUACaaaaAC-CGUUCUCUGC- -5' |
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| 21920 | 5' | -47.2 | NC_004913.1 | + | 26259 | 1.09 | 0.007955 |
|
Target: 5'- uCACCGUCAUGUUUUUGGCAAGAGACGc -3' miRNA: 3'- -GUGGCAGUACAAAAACCGUUCUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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