Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21921 | 3' | -57.5 | NC_004913.1 | + | 45671 | 0.66 | 0.608743 |
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Target: 5'- uUCCGUCGCuucagucaUGCGGUUGauGGCGuAGCg -3' miRNA: 3'- uGGGCGGUGcg------ACGCCAAU--UCGC-UCG- -5' |
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| 21921 | 3' | -57.5 | NC_004913.1 | + | 56574 | 0.66 | 0.576467 |
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Target: 5'- uACCgGUUugGCUGuCGGUgccuuucuGUGGGCu -3' miRNA: 3'- -UGGgCGGugCGAC-GCCAauu-----CGCUCG- -5' |
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| 21921 | 3' | -57.5 | NC_004913.1 | + | 6216 | 0.67 | 0.534085 |
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Target: 5'- cCCCGUCACGCUGUGu---GGUGuacGGCu -3' miRNA: 3'- uGGGCGGUGCGACGCcaauUCGC---UCG- -5' |
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| 21921 | 3' | -57.5 | NC_004913.1 | + | 1631 | 0.68 | 0.503036 |
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Target: 5'- aGCCCGCUcugAUGCcuaUGCGGaacaaaucaAGGCGGGCc -3' miRNA: 3'- -UGGGCGG---UGCG---ACGCCaa-------UUCGCUCG- -5' |
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| 21921 | 3' | -57.5 | NC_004913.1 | + | 37872 | 0.71 | 0.34562 |
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Target: 5'- gGCCUGCauUAUGUcGUGGUUGAGCGGGa -3' miRNA: 3'- -UGGGCG--GUGCGaCGCCAAUUCGCUCg -5' |
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| 21921 | 3' | -57.5 | NC_004913.1 | + | 26490 | 1.1 | 0.000549 |
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Target: 5'- cACCCGCCACGCUGCGGUUAAGCGAGCc -3' miRNA: 3'- -UGGGCGGUGCGACGCCAAUUCGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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