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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21922 | 5' | -58.8 | NC_004913.1 | + | 246 | 0.66 | 0.512492 |
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Target: 5'- aUCACCa--C-CCACGCCUUCAGCu- -3' miRNA: 3'- cAGUGGgcgGcGGUGUGGAAGUCGuc -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 8651 | 0.66 | 0.543649 |
|
Target: 5'- cUCuCCUGCgccaGCCugGCCUuuUCAGCAu -3' miRNA: 3'- cAGuGGGCGg---CGGugUGGA--AGUCGUc -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 9671 | 0.67 | 0.442031 |
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Target: 5'- gGUCGCCCccaccggGCCuGCCGCACCUguuucuccuuUCGGUc- -3' miRNA: 3'- -CAGUGGG-------CGG-CGGUGUGGA----------AGUCGuc -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 19716 | 0.7 | 0.329504 |
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Target: 5'- aUCACCCaCCuuucuaGCCACACCgcCAGCAa -3' miRNA: 3'- cAGUGGGcGG------CGGUGUGGaaGUCGUc -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 20553 | 0.69 | 0.353695 |
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Target: 5'- cGUCACCCucuGuuGCCAUAuCCgcaUCAGCAu -3' miRNA: 3'- -CAGUGGG---CggCGGUGU-GGa--AGUCGUc -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 21079 | 0.68 | 0.387855 |
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Target: 5'- ---uUCCGuuGCCACAUCUUCAgagaacGCAGg -3' miRNA: 3'- caguGGGCggCGGUGUGGAAGU------CGUC- -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 21144 | 0.68 | 0.414859 |
|
Target: 5'- cUUGCCCGUgGCaaCGCGCCgaCGGCAGa -3' miRNA: 3'- cAGUGGGCGgCG--GUGUGGaaGUCGUC- -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 26673 | 1.07 | 0.000596 |
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Target: 5'- gGUCACCCGCCGCCACACCUUCAGCAGu -3' miRNA: 3'- -CAGUGGGCGGCGGUGUGGAAGUCGUC- -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 35933 | 0.67 | 0.492135 |
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Target: 5'- -aCACCaaagCGCCGCCAUACgguauugCAGCGGc -3' miRNA: 3'- caGUGG----GCGGCGGUGUGgaa----GUCGUC- -5' |
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| 21922 | 5' | -58.8 | NC_004913.1 | + | 59001 | 0.7 | 0.321716 |
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Target: 5'- uUCACCCGggcCCG-CGCACUUUCAGUAa -3' miRNA: 3'- cAGUGGGC---GGCgGUGUGGAAGUCGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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