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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21924 | 5' | -41.5 | NC_004913.1 | + | 24772 | 0.66 | 0.999989 |
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Target: 5'- aAACUGGCGGcaaaGUGGACgacaguAACAAc- -3' miRNA: 3'- -UUGACCGCCaa--UAUUUGau----UUGUUua -5' |
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| 21924 | 5' | -41.5 | NC_004913.1 | + | 56264 | 0.66 | 0.999984 |
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Target: 5'- cGCUGGUGGaUUAaGGACgguGGCAGAg -3' miRNA: 3'- uUGACCGCC-AAUaUUUGau-UUGUUUa -5' |
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| 21924 | 5' | -41.5 | NC_004913.1 | + | 50355 | 0.66 | 0.999984 |
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Target: 5'- uGCUGGgGGgcAUGGGCg--GCAAAUa -3' miRNA: 3'- uUGACCgCCaaUAUUUGauuUGUUUA- -5' |
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| 21924 | 5' | -41.5 | NC_004913.1 | + | 23552 | 0.66 | 0.999978 |
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Target: 5'- cGCUGGCGG--GUGAAUUGuuCGAu- -3' miRNA: 3'- uUGACCGCCaaUAUUUGAUuuGUUua -5' |
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| 21924 | 5' | -41.5 | NC_004913.1 | + | 16101 | 0.66 | 0.999968 |
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Target: 5'- uGGCUGGCGGacgcUGGGCUGAguuccauacgGCAAGg -3' miRNA: 3'- -UUGACCGCCaau-AUUUGAUU----------UGUUUa -5' |
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| 21924 | 5' | -41.5 | NC_004913.1 | + | 19627 | 0.7 | 0.998407 |
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Target: 5'- uGCUGGCGGUgu--GGCUAGAaAGGUg -3' miRNA: 3'- uUGACCGCCAauauUUGAUUUgUUUA- -5' |
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| 21924 | 5' | -41.5 | NC_004913.1 | + | 31361 | 0.76 | 0.936958 |
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Target: 5'- uAGCUGGCGGUcgggUGUcAACUGAGCcAAAUa -3' miRNA: 3'- -UUGACCGCCA----AUAuUUGAUUUG-UUUA- -5' |
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| 21924 | 5' | -41.5 | NC_004913.1 | + | 31418 | 1.05 | 0.044964 |
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Target: 5'- uAACUGGCGGUUAUAAACUAAACAAAUa -3' miRNA: 3'- -UUGACCGCCAAUAUUUGAUUUGUUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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