Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21926 | 3' | -48.3 | NC_004913.1 | + | 39472 | 0.66 | 0.982897 |
Target: 5'- ----uUGAGGcucaGAGCUGGACGCGUCa- -3' miRNA: 3'- uguuuGCUUC----UUCGGCUUGCGUAGag -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 39175 | 0.66 | 0.978082 |
Target: 5'- gGCAGG-GAAGcuGGCUGAACGUGUCg- -3' miRNA: 3'- -UGUUUgCUUCu-UCGGCUUGCGUAGag -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 36066 | 0.66 | 0.972333 |
Target: 5'- aACAAAgu--GAAGCCGAGCGCGccgCUg -3' miRNA: 3'- -UGUUUgcuuCUUCGGCUUGCGUa--GAg -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 37603 | 0.67 | 0.968401 |
Target: 5'- aAUAAACGggG-AGCUGuaugcacaaaGCAUCUCc -3' miRNA: 3'- -UGUUUGCuuCuUCGGCuug-------CGUAGAG- -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 24030 | 0.67 | 0.965568 |
Target: 5'- cCGcgUGAGGAuGGCgCGGGCGCAUCg- -3' miRNA: 3'- uGUuuGCUUCU-UCG-GCUUGCGUAGag -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 4113 | 0.67 | 0.961783 |
Target: 5'- uACGGAUGAGcGAAGCCac-CGCGUCa- -3' miRNA: 3'- -UGUUUGCUU-CUUCGGcuuGCGUAGag -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 44458 | 0.69 | 0.893344 |
Target: 5'- uACAagGACGcAGAAGCU--GCGCAUCUg -3' miRNA: 3'- -UGU--UUGCuUCUUCGGcuUGCGUAGAg -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 18080 | 0.75 | 0.627648 |
Target: 5'- gGCuGGCGAucaGGAGGCUGcAACGCGUCUg -3' miRNA: 3'- -UGuUUGCU---UCUUCGGC-UUGCGUAGAg -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 38689 | 0.77 | 0.536122 |
Target: 5'- gACAAG-GAuGAAGCCGAGCGCAUUg- -3' miRNA: 3'- -UGUUUgCUuCUUCGGCUUGCGUAGag -5' |
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21926 | 3' | -48.3 | NC_004913.1 | + | 41844 | 1.1 | 0.004548 |
Target: 5'- cACAAACGAAGAAGCCGAACGCAUCUCu -3' miRNA: 3'- -UGUUUGCUUCUUCGGCUUGCGUAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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