Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21927 | 3' | -49.3 | NC_004913.1 | + | 11220 | 0.67 | 0.936888 |
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Target: 5'- uACAGACAG-GAGCUgucgUCGgcgcggggauccCCAGACg -3' miRNA: 3'- cUGUUUGUCuCUCGGaa--AGU------------GGUCUG- -5' |
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| 21927 | 3' | -49.3 | NC_004913.1 | + | 5300 | 0.67 | 0.931243 |
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Target: 5'- aAUGAACAGGGAGagaUUgucCACCAGGCc -3' miRNA: 3'- cUGUUUGUCUCUCgg-AAa--GUGGUCUG- -5' |
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| 21927 | 3' | -49.3 | NC_004913.1 | + | 8610 | 0.67 | 0.925291 |
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Target: 5'- aGCGGGCAGAGGcuGCCacugUCagugcugaagagGCCAGACg -3' miRNA: 3'- cUGUUUGUCUCU--CGGaa--AG------------UGGUCUG- -5' |
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| 21927 | 3' | -49.3 | NC_004913.1 | + | 8403 | 0.68 | 0.9056 |
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Target: 5'- cGGCAAccaGCAGGGcGGCUgaa-GCCGGACa -3' miRNA: 3'- -CUGUU---UGUCUC-UCGGaaagUGGUCUG- -5' |
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| 21927 | 3' | -49.3 | NC_004913.1 | + | 11684 | 0.68 | 0.883211 |
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Target: 5'- cGACAAAgGGGGAGCCUuauuugUUCAgUGGGg -3' miRNA: 3'- -CUGUUUgUCUCUCGGA------AAGUgGUCUg -5' |
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| 21927 | 3' | -49.3 | NC_004913.1 | + | 57341 | 0.7 | 0.791353 |
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Target: 5'- cGACAAguacACAGAG-GCUcUUCACCAG-Cg -3' miRNA: 3'- -CUGUU----UGUCUCuCGGaAAGUGGUCuG- -5' |
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| 21927 | 3' | -49.3 | NC_004913.1 | + | 59730 | 0.72 | 0.72671 |
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Target: 5'- uGACuGGCuGAGAGCCUcaUUCACCAu-- -3' miRNA: 3'- -CUGuUUGuCUCUCGGA--AAGUGGUcug -5' |
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| 21927 | 3' | -49.3 | NC_004913.1 | + | 45929 | 1.09 | 0.003695 |
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Target: 5'- uGACAAACAGAGAGCCUUUCACCAGACc -3' miRNA: 3'- -CUGUUUGUCUCUCGGAAAGUGGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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