Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21928 | 5' | -52.3 | NC_004913.1 | + | 47991 | 0.66 | 0.873815 |
Target: 5'- -gAUGUuucGCGUCAGGUgcuAUGGCAugGUCa -3' miRNA: 3'- caUGCG---UGCGGUUCG---UACUGUugCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 45575 | 0.66 | 0.873815 |
Target: 5'- -aACGCuACGCCAucaaccGCAUGACugaaGCg -3' miRNA: 3'- caUGCG-UGCGGUu-----CGUACUGuug-CGg -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 6356 | 0.66 | 0.873016 |
Target: 5'- aGU-CGCACGCUguucccacgucuuGAGCcggugcauguUGACAGCGCg -3' miRNA: 3'- -CAuGCGUGCGG-------------UUCGu---------ACUGUUGCGg -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 25115 | 0.66 | 0.865716 |
Target: 5'- uUACGUacACGCU--GCAUGAUuucCGCCg -3' miRNA: 3'- cAUGCG--UGCGGuuCGUACUGuu-GCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 2612 | 0.66 | 0.857364 |
Target: 5'- gGUGCG-ACGUCAGGCGUGGCuaucaGUa -3' miRNA: 3'- -CAUGCgUGCGGUUCGUACUGuug--CGg -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 42219 | 0.66 | 0.857364 |
Target: 5'- --cUGCACGCUAAcGUuUGACuuUGCCa -3' miRNA: 3'- cauGCGUGCGGUU-CGuACUGuuGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 54275 | 0.66 | 0.857364 |
Target: 5'- -cGCGUuuaGCGCCAcucuucccGCAUGGCGcaaaauCGCCg -3' miRNA: 3'- caUGCG---UGCGGUu-------CGUACUGUu-----GCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 21131 | 0.67 | 0.830873 |
Target: 5'- gGUACagGCcCGCCuuGCccGUGGCAACGCg -3' miRNA: 3'- -CAUG--CGuGCGGuuCG--UACUGUUGCGg -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 30358 | 0.67 | 0.830873 |
Target: 5'- -aACGC-CG-UAAGCAggaGCAACGCCa -3' miRNA: 3'- caUGCGuGCgGUUCGUac-UGUUGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 19989 | 0.67 | 0.821595 |
Target: 5'- gGUGCGCGCGC--AGC-UGGCAACcgauCCu -3' miRNA: 3'- -CAUGCGUGCGguUCGuACUGUUGc---GG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 20290 | 0.67 | 0.821595 |
Target: 5'- gGUGCGguUGCCGGauauCGUGGCAgACGUCa -3' miRNA: 3'- -CAUGCguGCGGUUc---GUACUGU-UGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 35926 | 0.67 | 0.821595 |
Target: 5'- -aGCGC-CGCCAuacGGUAUuGCAGCGgCa -3' miRNA: 3'- caUGCGuGCGGU---UCGUAcUGUUGCgG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 26155 | 0.67 | 0.81211 |
Target: 5'- --uUGuCACGCC-AGCA-GGCGGCGUCu -3' miRNA: 3'- cauGC-GUGCGGuUCGUaCUGUUGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 32094 | 0.67 | 0.81211 |
Target: 5'- ---aGCACGCauagguauGGCGUGACugauACGCUu -3' miRNA: 3'- caugCGUGCGgu------UCGUACUGu---UGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 36254 | 0.67 | 0.80145 |
Target: 5'- -cAUGCAggcUGCCAuuaucgaauggcaGGCAacUGGCGACGCUa -3' miRNA: 3'- caUGCGU---GCGGU-------------UCGU--ACUGUUGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 8120 | 0.68 | 0.790568 |
Target: 5'- -gACGCAcuggugcCGCUGAGCGcauccggugaaacUGuCAGCGCCg -3' miRNA: 3'- caUGCGU-------GCGGUUCGU-------------ACuGUUGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 7315 | 0.68 | 0.782521 |
Target: 5'- aGUACGgGCuugucGCCAGuGUAUGACGauAUGCCc -3' miRNA: 3'- -CAUGCgUG-----CGGUU-CGUACUGU--UGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 56065 | 0.7 | 0.641595 |
Target: 5'- aUGCGaccggguuaACGCCAuugAGCGUGAUAagGCGCUg -3' miRNA: 3'- cAUGCg--------UGCGGU---UCGUACUGU--UGCGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 26236 | 0.71 | 0.607938 |
Target: 5'- -gACGC-CGCCugcuGGCGUGACAAa-CCg -3' miRNA: 3'- caUGCGuGCGGu---UCGUACUGUUgcGG- -5' |
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21928 | 5' | -52.3 | NC_004913.1 | + | 21304 | 0.71 | 0.574475 |
Target: 5'- -cGCGUACGCCGgaugaaaaucaGGCcgGACGugGUg -3' miRNA: 3'- caUGCGUGCGGU-----------UCGuaCUGUugCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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