Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21929 | 3' | -53.3 | NC_004913.1 | + | 35820 | 0.66 | 0.790972 |
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Target: 5'- ----cUCCuGGCgucuGGCAGUGGGUGAgacUCAg -3' miRNA: 3'- aauuuAGG-CCG----CCGUUACCCACU---GGU- -5' |
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| 21929 | 3' | -53.3 | NC_004913.1 | + | 9584 | 0.66 | 0.780852 |
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Target: 5'- -cAGGUgCGGCaGGCccGGUGGGggcGACCGg -3' miRNA: 3'- aaUUUAgGCCG-CCG--UUACCCa--CUGGU- -5' |
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| 21929 | 3' | -53.3 | NC_004913.1 | + | 9350 | 0.67 | 0.760139 |
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Target: 5'- -cAGGUCCGGCugGGcCGAaGGGUGAUa- -3' miRNA: 3'- aaUUUAGGCCG--CC-GUUaCCCACUGgu -5' |
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| 21929 | 3' | -53.3 | NC_004913.1 | + | 25956 | 0.71 | 0.527522 |
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Target: 5'- -gAAAaCCGGCuGGUuuaacAUGGGUGACCu -3' miRNA: 3'- aaUUUaGGCCG-CCGu----UACCCACUGGu -5' |
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| 21929 | 3' | -53.3 | NC_004913.1 | + | 26594 | 0.71 | 0.527522 |
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Target: 5'- -aAGGUgUGGCGGC---GGGUGACCu -3' miRNA: 3'- aaUUUAgGCCGCCGuuaCCCACUGGu -5' |
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| 21929 | 3' | -53.3 | NC_004913.1 | + | 24989 | 0.71 | 0.495334 |
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Target: 5'- -aAAAUgCCGGaCGGCGGUucgGGGaUGACCAa -3' miRNA: 3'- aaUUUA-GGCC-GCCGUUA---CCC-ACUGGU- -5' |
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| 21929 | 3' | -53.3 | NC_004913.1 | + | 20029 | 0.74 | 0.35059 |
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Target: 5'- gUGAAUgCCauGGCGGCAAaacUGGGUGACgCAc -3' miRNA: 3'- aAUUUA-GG--CCGCCGUU---ACCCACUG-GU- -5' |
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| 21929 | 3' | -53.3 | NC_004913.1 | + | 50482 | 1.06 | 0.00214 |
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Target: 5'- cUUAAAUCCGGCGGCAAUGGGUGACCAg -3' miRNA: 3'- -AAUUUAGGCCGCCGUUACCCACUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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