Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21932 | 5' | -52.1 | NC_004913.1 | + | 2111 | 0.66 | 0.80145 |
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Target: 5'- gCGGUAGCUguggCAUCCGGGcgGGU-UCAGg -3' miRNA: 3'- -GUCGUCGA----GUAGGUCUuaCCGuAGUU- -5' |
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| 21932 | 5' | -52.1 | NC_004913.1 | + | 2420 | 0.67 | 0.770431 |
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Target: 5'- gAGCAGaucgaaCGUCCGGA-UGGCAUUAu -3' miRNA: 3'- gUCGUCga----GUAGGUCUuACCGUAGUu -5' |
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| 21932 | 5' | -52.1 | NC_004913.1 | + | 15069 | 0.69 | 0.658849 |
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Target: 5'- cCGGCAGCUUcugCCAGAAcGGaUAUCAGg -3' miRNA: 3'- -GUCGUCGAGua-GGUCUUaCC-GUAGUU- -5' |
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| 21932 | 5' | -52.1 | NC_004913.1 | + | 45308 | 0.69 | 0.6358 |
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Target: 5'- aCAGCAGUgagcgCGUCCAGAAaguucUGGCGa--- -3' miRNA: 3'- -GUCGUCGa----GUAGGUCUU-----ACCGUaguu -5' |
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| 21932 | 5' | -52.1 | NC_004913.1 | + | 55461 | 0.76 | 0.271333 |
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Target: 5'- cCGGCAGCUCcggagCCGGAA-GGCAUCGc -3' miRNA: 3'- -GUCGUCGAGua---GGUCUUaCCGUAGUu -5' |
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| 21932 | 5' | -52.1 | NC_004913.1 | + | 55514 | 1.06 | 0.002607 |
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Target: 5'- cCAGCAGCUCAUCCAGAAUGGCAUCAAc -3' miRNA: 3'- -GUCGUCGAGUAGGUCUUACCGUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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