miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21936 3' -57.4 NC_004913.1 + 8895 0.68 0.511688
Target:  5'- gGGGGCCUaaaggggacCAGGGGGAua-UGGggCCa- -3'
miRNA:   3'- -CCCCGGA---------GUCCCUCUuugACCa-GGcu -5'
21936 3' -57.4 NC_004913.1 + 9341 0.68 0.521954
Target:  5'- cGGGGCCagCAGGucc-GGCUGGgCCGAa -3'
miRNA:   3'- -CCCCGGa-GUCCcucuUUGACCaGGCU- -5'
21936 3' -57.4 NC_004913.1 + 9104 0.7 0.423676
Target:  5'- aGGGaCCUCGuGGAGAGcCaGGUCCGGc -3'
miRNA:   3'- cCCC-GGAGUcCCUCUUuGaCCAGGCU- -5'
21936 3' -57.4 NC_004913.1 + 9421 0.72 0.284939
Target:  5'- cGGGGaCCggCAGGu-GAuGCUGGUCCGGc -3'
miRNA:   3'- -CCCC-GGa-GUCCcuCUuUGACCAGGCU- -5'
21936 3' -57.4 NC_004913.1 + 8797 0.72 0.299223
Target:  5'- gGGGGCUgUAGGGccu-GCUGGUCCGc -3'
miRNA:   3'- -CCCCGGaGUCCCucuuUGACCAGGCu -5'
21936 3' -57.4 NC_004913.1 + 9475 0.78 0.130312
Target:  5'- aGGGGagagCGGGGAGAGACcGGUCUGAc -3'
miRNA:   3'- -CCCCgga-GUCCCUCUUUGaCCAGGCU- -5'
21936 3' -57.4 NC_004913.1 + 8679 1.11 0.000559
Target:  5'- gGGGGCCUCAGGGAGAAACUGGUCCGAa -3'
miRNA:   3'- -CCCCGGAGUCCCUCUUUGACCAGGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.