Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21937 | 3' | -59.5 | NC_004913.1 | + | 19563 | 0.67 | 0.435283 |
Target: 5'- aUGaUUCUGC-GGUCUGGGuGGCGAAg -3' miRNA: 3'- gACcAGGACGuCCAGGCUCuCCGCUU- -5' |
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21937 | 3' | -59.5 | NC_004913.1 | + | 5080 | 0.67 | 0.432452 |
Target: 5'- aCUGGUCCUGCAGGUcauuaaaguacgucCCcAGAaGCGu- -3' miRNA: 3'- -GACCAGGACGUCCA--------------GGcUCUcCGCuu -5' |
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21937 | 3' | -59.5 | NC_004913.1 | + | 11734 | 0.68 | 0.386917 |
Target: 5'- uCUGGUUCcaaaaucccuucaaUGCAGGcgugUCUGAGcAGGCGGAg -3' miRNA: 3'- -GACCAGG--------------ACGUCC----AGGCUC-UCCGCUU- -5' |
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21937 | 3' | -59.5 | NC_004913.1 | + | 8953 | 0.68 | 0.380799 |
Target: 5'- -aGGUCCUGCAGGcCCGcaAGGUc-- -3' miRNA: 3'- gaCCAGGACGUCCaGGCucUCCGcuu -5' |
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21937 | 3' | -59.5 | NC_004913.1 | + | 9585 | 0.68 | 0.380799 |
Target: 5'- -aGGUgCgGCAGGcCCGguGGGGGCGAc -3' miRNA: 3'- gaCCAgGaCGUCCaGGC--UCUCCGCUu -5' |
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21937 | 3' | -59.5 | NC_004913.1 | + | 8806 | 0.68 | 0.355319 |
Target: 5'- -aGGgCCUGCuGGUCCGcGuGGUGAGa -3' miRNA: 3'- gaCCaGGACGuCCAGGCuCuCCGCUU- -5' |
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21937 | 3' | -59.5 | NC_004913.1 | + | 9439 | 0.77 | 0.083917 |
Target: 5'- gCUGGUCCgGCAGGcCCGcAGGGGcCGAAa -3' miRNA: 3'- -GACCAGGaCGUCCaGGC-UCUCC-GCUU- -5' |
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21937 | 3' | -59.5 | NC_004913.1 | + | 9058 | 1.05 | 0.000677 |
Target: 5'- cCUGGUCCUGCAGGUCCGAGAGGCGAAc -3' miRNA: 3'- -GACCAGGACGUCCAGGCUCUCCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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