Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21941 | 5' | -55 | NC_004913.1 | + | 24665 | 0.67 | 0.67271 |
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Target: 5'- -cUCCCguaccaGCAGUCUgacaccggaaGAGACAAUUGCAUc -3' miRNA: 3'- guAGGG------CGUCGGA----------CUCUGUUAGCGUG- -5' |
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| 21941 | 5' | -55 | NC_004913.1 | + | 48749 | 0.67 | 0.650596 |
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Target: 5'- gUAUCCaaaGCGGCaUUGAuGGCGAUCGgGCu -3' miRNA: 3'- -GUAGGg--CGUCG-GACU-CUGUUAGCgUG- -5' |
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| 21941 | 5' | -55 | NC_004913.1 | + | 6684 | 0.68 | 0.606244 |
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Target: 5'- gUAUCCgGguGCgUGAGGCAuuUCGC-Cg -3' miRNA: 3'- -GUAGGgCguCGgACUCUGUu-AGCGuG- -5' |
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| 21941 | 5' | -55 | NC_004913.1 | + | 23454 | 0.71 | 0.447253 |
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Target: 5'- gGUCCCGCAGgUUGAaGCGGUUGCcCg -3' miRNA: 3'- gUAGGGCGUCgGACUcUGUUAGCGuG- -5' |
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| 21941 | 5' | -55 | NC_004913.1 | + | 18732 | 0.71 | 0.412486 |
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Target: 5'- uCAUCcaCCGCAGCCUGAG-CAuuaccgccauucuugAUCGCGu -3' miRNA: 3'- -GUAG--GGCGUCGGACUCuGU---------------UAGCGUg -5' |
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| 21941 | 5' | -55 | NC_004913.1 | + | 21640 | 1.09 | 0.001052 |
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Target: 5'- aCAUCCCGCAGCCUGAGACAAUCGCACc -3' miRNA: 3'- -GUAGGGCGUCGGACUCUGUUAGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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