miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21942 3' -52 NC_004913.1 + 55882 0.66 0.897901
Target:  5'- cCGuUCUGcGcUGAuugccgcagGUGCUUCUGCGCCUg -3'
miRNA:   3'- -GCuAGAC-CuACU---------CAUGGAGAUGCGGG- -5'
21942 3' -52 NC_004913.1 + 44681 0.69 0.755207
Target:  5'- gCGAUCUGGuuUGAGU-CCUgUuucaaACGCCa -3'
miRNA:   3'- -GCUAGACCu-ACUCAuGGAgA-----UGCGGg -5'
21942 3' -52 NC_004913.1 + 55438 0.69 0.74474
Target:  5'- --uUCUGGAUGAGcUGCUgg-GCGCaCCg -3'
miRNA:   3'- gcuAGACCUACUC-AUGGagaUGCG-GG- -5'
21942 3' -52 NC_004913.1 + 24919 1.14 0.001273
Target:  5'- cCGAUCUGGAUGAGUACCUCUACGCCCg -3'
miRNA:   3'- -GCUAGACCUACUCAUGGAGAUGCGGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.