Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21943 | 3' | -42.6 | NC_004913.1 | + | 20625 | 0.67 | 0.999754 |
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Target: 5'- -aCUCAgCCAGAAAGaggGGCGCAc-- -3' miRNA: 3'- cgGAGUaGGUCUUUUaa-UUGCGUuua -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 2384 | 0.67 | 0.999754 |
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Target: 5'- aCCUCAUCCugugccGGAA--UGGCGCGGGa -3' miRNA: 3'- cGGAGUAGGu-----CUUUuaAUUGCGUUUa -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 3067 | 0.69 | 0.998627 |
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Target: 5'- gGCa-CAUCCAGAAGAUUAACccaCAGGUc -3' miRNA: 3'- -CGgaGUAGGUCUUUUAAUUGc--GUUUA- -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 7823 | 0.69 | 0.998299 |
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Target: 5'- aGCCUCuUCCAGuGGAAcUGGCGUAu-- -3' miRNA: 3'- -CGGAGuAGGUC-UUUUaAUUGCGUuua -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 53134 | 0.69 | 0.998299 |
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Target: 5'- aGCaCUUAUUCAGgcGAUUGuCGCAGAa -3' miRNA: 3'- -CG-GAGUAGGUCuuUUAAUuGCGUUUa -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 23252 | 0.69 | 0.997906 |
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Target: 5'- cGCCUUAUCCuGAAca-UAACGCu--- -3' miRNA: 3'- -CGGAGUAGGuCUUuuaAUUGCGuuua -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 56006 | 0.69 | 0.997906 |
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Target: 5'- uUUUCAUCCAGAAacuggucgagGAUUucaGGCGCAGAa -3' miRNA: 3'- cGGAGUAGGUCUU----------UUAA---UUGCGUUUa -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 159 | 0.7 | 0.995501 |
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Target: 5'- cGCCUUAgCCGGAuauUUcGCGCAAAUu -3' miRNA: 3'- -CGGAGUaGGUCUuuuAAuUGCGUUUA- -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 24991 | 0.72 | 0.985851 |
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Target: 5'- -aCUCAUCCAGAucggccuGCGCAAu- -3' miRNA: 3'- cgGAGUAGGUCUuuuaau-UGCGUUua -5' |
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| 21943 | 3' | -42.6 | NC_004913.1 | + | 31602 | 1.09 | 0.021967 |
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Target: 5'- uGCCUCAUCCAGAAAAUUAACGCAAAUu -3' miRNA: 3'- -CGGAGUAGGUCUUUUAAUUGCGUUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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