Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21944 | 3' | -49.4 | NC_004913.1 | + | 21229 | 0.66 | 0.964175 |
Target: 5'- uGCCGucgGCGcguUGCCACGgGCAagGCg- -3' miRNA: 3'- -CGGCua-UGU---ACGGUGUgCGUaaUGag -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 37914 | 0.66 | 0.964175 |
Target: 5'- uGCCGGUACuuUGCCAacuaugaccCACGUAUgaaGCg- -3' miRNA: 3'- -CGGCUAUGu-ACGGU---------GUGCGUAa--UGag -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 6381 | 0.66 | 0.951766 |
Target: 5'- aGCCGGUGCAUGUUGacaGCGCGa----- -3' miRNA: 3'- -CGGCUAUGUACGGUg--UGCGUaaugag -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 12730 | 0.66 | 0.951766 |
Target: 5'- uGCUGAUGCAgugcugguUGCCACgACGCcgggGCa- -3' miRNA: 3'- -CGGCUAUGU--------ACGGUG-UGCGuaa-UGag -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 19980 | 0.66 | 0.951766 |
Target: 5'- uGCCGAUGagGUG-CGCGCGCA--GCUg -3' miRNA: 3'- -CGGCUAUg-UACgGUGUGCGUaaUGAg -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 39262 | 0.67 | 0.925268 |
Target: 5'- uCCGGUACAUGgUugAaacCAUUGCUCa -3' miRNA: 3'- cGGCUAUGUACgGugUgc-GUAAUGAG- -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 54557 | 0.68 | 0.91907 |
Target: 5'- gGCCGGUAUcuucacUGCCACAgGgCGUUGCc- -3' miRNA: 3'- -CGGCUAUGu-----ACGGUGUgC-GUAAUGag -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 14127 | 0.68 | 0.905778 |
Target: 5'- gGCCGAUGuCAUG-UACACGCAUg---- -3' miRNA: 3'- -CGGCUAU-GUACgGUGUGCGUAaugag -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 50019 | 0.68 | 0.898688 |
Target: 5'- uGCUGGU---UGCaCAcCACGCAUUGCUUg -3' miRNA: 3'- -CGGCUAuguACG-GU-GUGCGUAAUGAG- -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 47428 | 0.72 | 0.740032 |
Target: 5'- cGCUGcuuauugGCAUGCCuuGCACGCGUUuacCUCg -3' miRNA: 3'- -CGGCua-----UGUACGG--UGUGCGUAAu--GAG- -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 36056 | 0.72 | 0.706782 |
Target: 5'- aGCCGA-GCGcGCCGCugGCAUUcGCaUCa -3' miRNA: 3'- -CGGCUaUGUaCGGUGugCGUAA-UG-AG- -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 54259 | 0.76 | 0.514164 |
Target: 5'- uCCGGUGCAgaaGCCACAgGCGUcacaacacUGCUCu -3' miRNA: 3'- cGGCUAUGUa--CGGUGUgCGUA--------AUGAG- -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 1598 | 0.77 | 0.460778 |
Target: 5'- uGCCGAUGCAUGCCGccUugGCAaUAUg- -3' miRNA: 3'- -CGGCUAUGUACGGU--GugCGUaAUGag -5' |
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21944 | 3' | -49.4 | NC_004913.1 | + | 35934 | 1.14 | 0.001983 |
Target: 5'- aGCCGAUACAUGCCACACGCAUUACUCa -3' miRNA: 3'- -CGGCUAUGUACGGUGUGCGUAAUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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