Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21949 | 5' | -51.3 | NC_004913.1 | + | 35893 | 0.66 | 0.902047 |
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Target: 5'- aGCAGCAAAGagauGCAGGAgagcaaaacgaUGAaaaaCCGUAa -3' miRNA: 3'- gCGUCGUUUCg---CGUCUU-----------ACU----GGCAUa -5' |
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| 21949 | 5' | -51.3 | NC_004913.1 | + | 27135 | 0.66 | 0.894818 |
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Target: 5'- gCGCAGUAucaCGCAGAGcugaagagucUGACCGg-- -3' miRNA: 3'- -GCGUCGUuucGCGUCUU----------ACUGGCaua -5' |
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| 21949 | 5' | -51.3 | NC_004913.1 | + | 18164 | 0.66 | 0.879505 |
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Target: 5'- uGCAGCGAAa-GCGGGucaGGCCGUGa -3' miRNA: 3'- gCGUCGUUUcgCGUCUua-CUGGCAUa -5' |
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| 21949 | 5' | -51.3 | NC_004913.1 | + | 17208 | 0.67 | 0.863089 |
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Target: 5'- gCGCAGau-GGCGCAGGAUaACCa--- -3' miRNA: 3'- -GCGUCguuUCGCGUCUUAcUGGcaua -5' |
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| 21949 | 5' | -51.3 | NC_004913.1 | + | 18623 | 0.72 | 0.564725 |
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Target: 5'- aGCGGCGGAcGCGaucaAGAAUGGCgGUAa -3' miRNA: 3'- gCGUCGUUU-CGCg---UCUUACUGgCAUa -5' |
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| 21949 | 5' | -51.3 | NC_004913.1 | + | 47948 | 1.07 | 0.003159 |
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Target: 5'- gCGCAGCAAAGCGCAGAAUGACCGUAUg -3' miRNA: 3'- -GCGUCGUUUCGCGUCUUACUGGCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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