miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2195 5' -51.2 NC_001396.1 + 706 0.66 0.330153
Target:  5'- gCGCAucaguUCCAGCccguuGCGCAcacgUGCGGG-Ca -3'
miRNA:   3'- gGUGUu----AGGUCGu----UGCGU----ACGCUCaG- -5'
2195 5' -51.2 NC_001396.1 + 4245 0.69 0.201526
Target:  5'- cCCACAuaaUAGCcguagaagccaaGGCGCGUGCGAGg- -3'
miRNA:   3'- -GGUGUuagGUCG------------UUGCGUACGCUCag -5'
2195 5' -51.2 NC_001396.1 + 7129 0.74 0.090378
Target:  5'- cUCGCGAUgguaagCCAGCAACGCAUuuccGCGAG-Ca -3'
miRNA:   3'- -GGUGUUA------GGUCGUUGCGUA----CGCUCaG- -5'
2195 5' -51.2 NC_001396.1 + 4569 1.12 0.000078
Target:  5'- cCCACAAUCCAGCAACGCAUGCGAGUCu -3'
miRNA:   3'- -GGUGUUAGGUCGUUGCGUACGCUCAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.