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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21950 | 3' | -55.4 | NC_004913.1 | + | 2103 | 0.66 | 0.721881 |
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Target: 5'- uGAUGCagGCGGuagcuGUGGCAuCCGGGCg -3' miRNA: 3'- -UUAUG--CGCCcaaacCACCGU-GGUCCG- -5' |
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| 21950 | 3' | -55.4 | NC_004913.1 | + | 17399 | 0.66 | 0.711127 |
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Target: 5'- uGUugGCGGccaacUGGUGGCAgCguaaggGGGCa -3' miRNA: 3'- uUAugCGCCcaa--ACCACCGUgG------UCCG- -5' |
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| 21950 | 3' | -55.4 | NC_004913.1 | + | 54190 | 0.66 | 0.711127 |
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Target: 5'- --cAUGCGGGaagaGUGGCGCUaaacgcggGGGCa -3' miRNA: 3'- uuaUGCGCCCaaacCACCGUGG--------UCCG- -5' |
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| 21950 | 3' | -55.4 | NC_004913.1 | + | 22432 | 0.67 | 0.656404 |
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Target: 5'- --aAUGCGGGUgagaGGUGGCuuuaagucaGCCAGugauGCg -3' miRNA: 3'- uuaUGCGCCCAaa--CCACCG---------UGGUC----CG- -5' |
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| 21950 | 3' | -55.4 | NC_004913.1 | + | 59080 | 0.67 | 0.656404 |
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Target: 5'- aAGUGCGCGGGcccgGGUGaaCACCAGu- -3' miRNA: 3'- -UUAUGCGCCCaaa-CCACc-GUGGUCcg -5' |
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| 21950 | 3' | -55.4 | NC_004913.1 | + | 56739 | 0.68 | 0.590083 |
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Target: 5'- ---uUGCGGGgaUGG-GGCugGCCuGGCa -3' miRNA: 3'- uuauGCGCCCaaACCaCCG--UGGuCCG- -5' |
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| 21950 | 3' | -55.4 | NC_004913.1 | + | 37823 | 0.74 | 0.260524 |
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Target: 5'- cAUACGUGGGUcauagUUGGcaaaGUACCAGGCa -3' miRNA: 3'- uUAUGCGCCCA-----AACCac--CGUGGUCCG- -5' |
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| 21950 | 3' | -55.4 | NC_004913.1 | + | 52670 | 1.09 | 0.000991 |
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Target: 5'- gAAUACGCGGGUUUGGUGGCACCAGGCc -3' miRNA: 3'- -UUAUGCGCCCAAACCACCGUGGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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