Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21950 | 5' | -56.1 | NC_004913.1 | + | 32104 | 0.66 | 0.655027 |
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Target: 5'- gGCACuCCaGCGCCA-GCAuggucuacaauguuuAUGGUGCAUg -3' miRNA: 3'- -CGUG-GGcCGUGGUaCGU---------------UACCGUGUA- -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 57667 | 0.67 | 0.606244 |
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Target: 5'- uGC-UCCGGUACCGUGCGugaagccaccGGCACc- -3' miRNA: 3'- -CGuGGGCCGUGGUACGUua--------CCGUGua -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 19813 | 0.67 | 0.584164 |
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Target: 5'- uGCACaggGGCAgaGUGCAAUGGCGgCAg -3' miRNA: 3'- -CGUGgg-CCGUggUACGUUACCGU-GUa -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 11411 | 0.67 | 0.584164 |
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Target: 5'- ---aCUGGCAUUcUGCAAUGGCACc- -3' miRNA: 3'- cgugGGCCGUGGuACGUUACCGUGua -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 24804 | 0.67 | 0.573181 |
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Target: 5'- aGCACCUGGaauuuauCC-UGCAucUGGCGCAc -3' miRNA: 3'- -CGUGGGCCgu-----GGuACGUu-ACCGUGUa -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 53325 | 0.68 | 0.498099 |
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Target: 5'- cGgACCCGcGCAUUAaGCAGcUGGCGCGUc -3' miRNA: 3'- -CgUGGGC-CGUGGUaCGUU-ACCGUGUA- -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 39753 | 0.69 | 0.477431 |
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Target: 5'- gGCAgCCagcaacaGGcCACCcUGCAAUGGCAUAUu -3' miRNA: 3'- -CGU-GGg------CC-GUGGuACGUUACCGUGUA- -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 46539 | 0.69 | 0.444294 |
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Target: 5'- gGCAauaCCGGCACgGUGUAuugcauucaggcaaAUGGCGCu- -3' miRNA: 3'- -CGUg--GGCCGUGgUACGU--------------UACCGUGua -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 36559 | 0.75 | 0.191535 |
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Target: 5'- -aACCCGGCuACCGUGC--UGGCACu- -3' miRNA: 3'- cgUGGGCCG-UGGUACGuuACCGUGua -5' |
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| 21950 | 5' | -56.1 | NC_004913.1 | + | 52711 | 1.08 | 0.000852 |
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Target: 5'- gGCACCCGGCACCAUGCAAUGGCACAUa -3' miRNA: 3'- -CGUGGGCCGUGGUACGUUACCGUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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