Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21954 | 5' | -44.9 | NC_004914.1 | + | 3756 | 0.66 | 0.998439 |
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Target: 5'- aGGACGUgaaggagCCGGAAGCGAAGGGg -3' miRNA: 3'- -CUUGCGaaugga-GGCCUUUGUUUUUU- -5' |
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| 21954 | 5' | -44.9 | NC_004914.1 | + | 27051 | 0.67 | 0.996694 |
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Target: 5'- aAGCGCccugucCCUCCGGAGAUAu---- -3' miRNA: 3'- cUUGCGaau---GGAGGCCUUUGUuuuuu -5' |
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| 21954 | 5' | -44.9 | NC_004914.1 | + | 26371 | 0.68 | 0.990911 |
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Target: 5'- -cGCGCUUGCCgcugUGGGAGCAGGu-- -3' miRNA: 3'- cuUGCGAAUGGag--GCCUUUGUUUuuu -5' |
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| 21954 | 5' | -44.9 | NC_004914.1 | + | 56365 | 0.68 | 0.990911 |
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Target: 5'- gGAAgGCUgggccauacuCCUCCGGcagAAGCAGAAAAa -3' miRNA: 3'- -CUUgCGAau--------GGAGGCC---UUUGUUUUUU- -5' |
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| 21954 | 5' | -44.9 | NC_004914.1 | + | 43949 | 0.69 | 0.987785 |
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Target: 5'- cAACGCUUACCcagCgCGGucuGGCAAAGAu -3' miRNA: 3'- cUUGCGAAUGGa--G-GCCu--UUGUUUUUu -5' |
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| 21954 | 5' | -44.9 | NC_004914.1 | + | 55399 | 0.7 | 0.973355 |
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Target: 5'- cGACGU--AUUUCUGGAAGCAGAAGAa -3' miRNA: 3'- cUUGCGaaUGGAGGCCUUUGUUUUUU- -5' |
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| 21954 | 5' | -44.9 | NC_004914.1 | + | 16064 | 0.71 | 0.958561 |
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Target: 5'- uGAGCGU-UACCUgCCGGAggUGAAGAc -3' miRNA: 3'- -CUUGCGaAUGGA-GGCCUuuGUUUUUu -5' |
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| 21954 | 5' | -44.9 | NC_004914.1 | + | 56079 | 0.71 | 0.954124 |
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Target: 5'- aGGGCGUUgcCCUgaCCGGAGACGGGGGGa -3' miRNA: 3'- -CUUGCGAauGGA--GGCCUUUGUUUUUU- -5' |
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| 21954 | 5' | -44.9 | NC_004914.1 | + | 3523 | 1.07 | 0.017474 |
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Target: 5'- gGAACGCUUACCUCCGGAAACAAAAAAc -3' miRNA: 3'- -CUUGCGAAUGGAGGCCUUUGUUUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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