Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21956 | 3' | -58.1 | NC_004914.1 | + | 46023 | 0.66 | 0.644755 |
Target: 5'- aCCCGAauauuuuuuaccuucCGGCgacaaaugACGCCGAUGAC-GGCu -3' miRNA: 3'- aGGGCU---------------GCCG--------UGUGGUUGCUGaCCGc -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 5315 | 0.66 | 0.629875 |
Target: 5'- gUCCUGuACGuCACGCCGcgucaguggaAUGACUGGUa -3' miRNA: 3'- -AGGGC-UGCcGUGUGGU----------UGCUGACCGc -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 37641 | 0.66 | 0.629875 |
Target: 5'- cCCUGAaaGCACA---GCGGCUGGCu -3' miRNA: 3'- aGGGCUgcCGUGUgguUGCUGACCGc -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 13544 | 0.66 | 0.629875 |
Target: 5'- uUUCCGuucugaauaACGGCACuCCGGauaACUGGCGa -3' miRNA: 3'- -AGGGC---------UGCCGUGuGGUUgc-UGACCGC- -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 58886 | 0.66 | 0.628812 |
Target: 5'- aCCCGcaccaucACGGUAUGCUGugGugUGGUu -3' miRNA: 3'- aGGGC-------UGCCGUGUGGUugCugACCGc -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 238 | 0.66 | 0.608631 |
Target: 5'- -gCUGACGGCACAggauuauaaauuCCAuuuuuACGcCUGGUGg -3' miRNA: 3'- agGGCUGCCGUGU------------GGU-----UGCuGACCGC- -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 59166 | 0.66 | 0.608631 |
Target: 5'- aUCCCGuCGugaccaggagaGCGCGCUGGCGuGCUGGgGa -3' miRNA: 3'- -AGGGCuGC-----------CGUGUGGUUGC-UGACCgC- -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 35568 | 0.67 | 0.566433 |
Target: 5'- -gCUGGCugugcaaaagaGGCGCGCaagGAUGGCUGGCGa -3' miRNA: 3'- agGGCUG-----------CCGUGUGg--UUGCUGACCGC- -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 55330 | 0.67 | 0.545602 |
Target: 5'- aCCUGGCggauauGGCAgGCCAguGCGucgguggcucuGCUGGCGg -3' miRNA: 3'- aGGGCUG------CCGUgUGGU--UGC-----------UGACCGC- -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 49909 | 0.68 | 0.514844 |
Target: 5'- gCCUGACGG---GCCGACGAaccuuCUGGCa -3' miRNA: 3'- aGGGCUGCCgugUGGUUGCU-----GACCGc -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 1845 | 0.68 | 0.491749 |
Target: 5'- uUCCCGGgaaUGGCucagguuaucgauuAUGCCAuugAUGACUGGCGu -3' miRNA: 3'- -AGGGCU---GCCG--------------UGUGGU---UGCUGACCGC- -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 55101 | 0.69 | 0.465266 |
Target: 5'- -gCCGAUGGCACAUaCAgugacGCGAgUGGUGc -3' miRNA: 3'- agGGCUGCCGUGUG-GU-----UGCUgACCGC- -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 57048 | 0.69 | 0.427452 |
Target: 5'- gCCCGu--GCGCAgcaCGACGACUGGCu -3' miRNA: 3'- aGGGCugcCGUGUg--GUUGCUGACCGc -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 38071 | 0.69 | 0.418286 |
Target: 5'- cUCCCGAUGGUuuauGCAgUGACGGCaaUGGCc -3' miRNA: 3'- -AGGGCUGCCG----UGUgGUUGCUG--ACCGc -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 60240 | 0.75 | 0.193384 |
Target: 5'- gUCCUGGCGGUugGCUAugGugcgcugugGCUGGCa -3' miRNA: 3'- -AGGGCUGCCGugUGGUugC---------UGACCGc -5' |
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21956 | 3' | -58.1 | NC_004914.1 | + | 6928 | 1.09 | 0.000645 |
Target: 5'- cUCCCGACGGCACACCAACGACUGGCGg -3' miRNA: 3'- -AGGGCUGCCGUGUGGUUGCUGACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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