miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21956 5' -54.6 NC_004914.1 + 25253 0.66 0.762246
Target:  5'- aACGGUAGgCGUucUGcCAG-GCAUCCa -3'
miRNA:   3'- gUGCCGUCgGUA--ACuGUCuCGUAGGc -5'
21956 5' -54.6 NC_004914.1 + 48756 0.66 0.741472
Target:  5'- aGCGGCauugauGGCgAUcgGGCAGGGCGUCa- -3'
miRNA:   3'- gUGCCG------UCGgUAa-CUGUCUCGUAGgc -5'
21956 5' -54.6 NC_004914.1 + 5758 0.66 0.74042
Target:  5'- uCACGGCGugauuGCCGUUGAUAcAGCAgugaagcUCUGa -3'
miRNA:   3'- -GUGCCGU-----CGGUAACUGUcUCGU-------AGGC- -5'
21956 5' -54.6 NC_004914.1 + 58784 0.67 0.707345
Target:  5'- aACGGCAGCCGauaaaccauaGCGGAGUuUCCu -3'
miRNA:   3'- gUGCCGUCGGUaac-------UGUCUCGuAGGc -5'
21956 5' -54.6 NC_004914.1 + 9589 0.7 0.523742
Target:  5'- uGCGGCAgGCCcg-GugGGGGCGaCCGg -3'
miRNA:   3'- gUGCCGU-CGGuaaCugUCUCGUaGGC- -5'
21956 5' -54.6 NC_004914.1 + 17601 0.71 0.47183
Target:  5'- gGgGGCGGCUAUUGGC---GCAUCCGu -3'
miRNA:   3'- gUgCCGUCGGUAACUGucuCGUAGGC- -5'
21956 5' -54.6 NC_004914.1 + 6893 1.08 0.001391
Target:  5'- cCACGGCAGCCAUUGACAGAGCAUCCGu -3'
miRNA:   3'- -GUGCCGUCGGUAACUGUCUCGUAGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.